Cargando…

Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway

BACKGROUND: Decidualization is essential to the successful pregnancy in mice. The molecular mechanisms and effects of Aurora kinase A (Aurora A) remain poorly understood during pregnancy. This study is the first to investigate the expression and role of Aurora A during mouse decidualization. METHODS...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Peng-Chao, Chen, Si-Ting, Yang, Zeng-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557062/
https://www.ncbi.nlm.nih.gov/pubmed/34715887
http://dx.doi.org/10.1186/s12958-021-00847-5
_version_ 1784592300354043904
author Wang, Peng-Chao
Chen, Si-Ting
Yang, Zeng-Ming
author_facet Wang, Peng-Chao
Chen, Si-Ting
Yang, Zeng-Ming
author_sort Wang, Peng-Chao
collection PubMed
description BACKGROUND: Decidualization is essential to the successful pregnancy in mice. The molecular mechanisms and effects of Aurora kinase A (Aurora A) remain poorly understood during pregnancy. This study is the first to investigate the expression and role of Aurora A during mouse decidualization. METHODS: Quantitative real time polymerase chain reaction, western blotting and in situ hybridization were used to determine the expression of Aurora A in mouse uteri. Aurora A activity was inhibited by Aurora A inhibitor to explore the role of Aurora A on decidualization via regulating the Aurora A/Stat3/Plk1/Cdk1 signaling pathway. RESULTS: Aurora A was strongly expressed at implantation sites compared with inter-implantation sites. Furthermore, Aurora A was also significantly increased in oil-induced deciduoma compared with control. Both Aurora A mRNA and protein were significantly increased under in vitro decidualization. Under in vitro decidualization, Prl8a2, a marker of mouse decidualization, was significantly decreased by TC-S 7010, an Aurora A inhibitor. Additionally, Prl8a2 was reduced by Stat3 inhibitor, Plk1 inhibitor and Cdk1 inhibitor, respectively. Moreover, the protein levels of p-Stat3, p-Plk1 and p-Cdk1 were suppressed by TC-S 7010. The protein levels of p-Stat3, p-Plk1 and p-Cdk1 were also suppressed by S3I-201, a Stat3 inhibitor). SBE 13 HCl (Plk1 inhibitor) could reduce the protein levels of p-Plk1 and p-Cdk1. Collectively, Aurora A could regulate Stat3/Plk1/Cdk1 signaling pathway. CONCLUSION: Our study shows that Aurora A is expressed in decidual cells and should be important for mouse decidualization. Aurora A/Stat3/Plk1/Cdk1 signaling pathway may be involved in mouse decidualization.
format Online
Article
Text
id pubmed-8557062
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-85570622021-11-01 Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway Wang, Peng-Chao Chen, Si-Ting Yang, Zeng-Ming Reprod Biol Endocrinol Research BACKGROUND: Decidualization is essential to the successful pregnancy in mice. The molecular mechanisms and effects of Aurora kinase A (Aurora A) remain poorly understood during pregnancy. This study is the first to investigate the expression and role of Aurora A during mouse decidualization. METHODS: Quantitative real time polymerase chain reaction, western blotting and in situ hybridization were used to determine the expression of Aurora A in mouse uteri. Aurora A activity was inhibited by Aurora A inhibitor to explore the role of Aurora A on decidualization via regulating the Aurora A/Stat3/Plk1/Cdk1 signaling pathway. RESULTS: Aurora A was strongly expressed at implantation sites compared with inter-implantation sites. Furthermore, Aurora A was also significantly increased in oil-induced deciduoma compared with control. Both Aurora A mRNA and protein were significantly increased under in vitro decidualization. Under in vitro decidualization, Prl8a2, a marker of mouse decidualization, was significantly decreased by TC-S 7010, an Aurora A inhibitor. Additionally, Prl8a2 was reduced by Stat3 inhibitor, Plk1 inhibitor and Cdk1 inhibitor, respectively. Moreover, the protein levels of p-Stat3, p-Plk1 and p-Cdk1 were suppressed by TC-S 7010. The protein levels of p-Stat3, p-Plk1 and p-Cdk1 were also suppressed by S3I-201, a Stat3 inhibitor). SBE 13 HCl (Plk1 inhibitor) could reduce the protein levels of p-Plk1 and p-Cdk1. Collectively, Aurora A could regulate Stat3/Plk1/Cdk1 signaling pathway. CONCLUSION: Our study shows that Aurora A is expressed in decidual cells and should be important for mouse decidualization. Aurora A/Stat3/Plk1/Cdk1 signaling pathway may be involved in mouse decidualization. BioMed Central 2021-10-29 /pmc/articles/PMC8557062/ /pubmed/34715887 http://dx.doi.org/10.1186/s12958-021-00847-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Peng-Chao
Chen, Si-Ting
Yang, Zeng-Ming
Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title_full Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title_fullStr Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title_full_unstemmed Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title_short Effects of Aurora kinase A on mouse decidualization via Stat3-plk1-cdk1 pathway
title_sort effects of aurora kinase a on mouse decidualization via stat3-plk1-cdk1 pathway
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557062/
https://www.ncbi.nlm.nih.gov/pubmed/34715887
http://dx.doi.org/10.1186/s12958-021-00847-5
work_keys_str_mv AT wangpengchao effectsofaurorakinaseaonmousedecidualizationviastat3plk1cdk1pathway
AT chensiting effectsofaurorakinaseaonmousedecidualizationviastat3plk1cdk1pathway
AT yangzengming effectsofaurorakinaseaonmousedecidualizationviastat3plk1cdk1pathway