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Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications

Recombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details...

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Detalles Bibliográficos
Autores principales:	Mital, Suraj, Christie, Graham, Dikicioglu, Duygu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557517/ https://www.ncbi.nlm.nih.gov/pubmed/34717620 http://dx.doi.org/10.1186/s12934-021-01698-w

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