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Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers

Sea turtles are threatened by climate change and human activity, and their global populations continue to decline sharply. The Chinese government encourages artificial breeding of sea turtles to reduce the use of wild populations. However, artificial breeding of sea turtles is still fairly difficult...

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Autores principales: Zhang, Ting, Lin, Liu, Gaillard, Daniel, Chen, Fang, Chen, Huaiqing, Li, Meimei, Wu, Shannan, Wang, Zhao, Shi, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557680/
https://www.ncbi.nlm.nih.gov/pubmed/34760392
http://dx.doi.org/10.7717/peerj.12410
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author Zhang, Ting
Lin, Liu
Gaillard, Daniel
Chen, Fang
Chen, Huaiqing
Li, Meimei
Wu, Shannan
Wang, Zhao
Shi, Haitao
author_facet Zhang, Ting
Lin, Liu
Gaillard, Daniel
Chen, Fang
Chen, Huaiqing
Li, Meimei
Wu, Shannan
Wang, Zhao
Shi, Haitao
author_sort Zhang, Ting
collection PubMed
description Sea turtles are threatened by climate change and human activity, and their global populations continue to decline sharply. The Chinese government encourages artificial breeding of sea turtles to reduce the use of wild populations. However, artificial breeding of sea turtles is still fairly difficult, and some facilities may illegally purchase wild turtle eggs and then sell incubated turtles by marketing them as artificially bred turtles, which adds another threat to an already endangered species. Therefore, it is necessary to find a reliable method to distinguish the authenticity of artificially bred individuals. In this study, we investigated a turtle farm in southern China, that contained more than 400 green turtles, which were claimed to have been bred in captivity. Parentage testing of turtles from this farm was successfully conducted using two nuclear microsatellites combined with a mitochondrial D-loop DNA marker. Genetic matching of all 19 adults and randomly selected 16 juvenile turtles revealed that none of the juvenile turtles had a matching parent combination among the adult turtles. Therefore, we speculated that the green turtles in this farm were from the wild and that their origin of birth was mainly the Sulu Sea. The methods and molecular markers used in this study could be a reference for rapid authenticity testing of green turtles in future forensic enforcement and population management.
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spelling pubmed-85576802021-11-09 Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers Zhang, Ting Lin, Liu Gaillard, Daniel Chen, Fang Chen, Huaiqing Li, Meimei Wu, Shannan Wang, Zhao Shi, Haitao PeerJ Conservation Biology Sea turtles are threatened by climate change and human activity, and their global populations continue to decline sharply. The Chinese government encourages artificial breeding of sea turtles to reduce the use of wild populations. However, artificial breeding of sea turtles is still fairly difficult, and some facilities may illegally purchase wild turtle eggs and then sell incubated turtles by marketing them as artificially bred turtles, which adds another threat to an already endangered species. Therefore, it is necessary to find a reliable method to distinguish the authenticity of artificially bred individuals. In this study, we investigated a turtle farm in southern China, that contained more than 400 green turtles, which were claimed to have been bred in captivity. Parentage testing of turtles from this farm was successfully conducted using two nuclear microsatellites combined with a mitochondrial D-loop DNA marker. Genetic matching of all 19 adults and randomly selected 16 juvenile turtles revealed that none of the juvenile turtles had a matching parent combination among the adult turtles. Therefore, we speculated that the green turtles in this farm were from the wild and that their origin of birth was mainly the Sulu Sea. The methods and molecular markers used in this study could be a reference for rapid authenticity testing of green turtles in future forensic enforcement and population management. PeerJ Inc. 2021-10-28 /pmc/articles/PMC8557680/ /pubmed/34760392 http://dx.doi.org/10.7717/peerj.12410 Text en ©2021 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Conservation Biology
Zhang, Ting
Lin, Liu
Gaillard, Daniel
Chen, Fang
Chen, Huaiqing
Li, Meimei
Wu, Shannan
Wang, Zhao
Shi, Haitao
Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title_full Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title_fullStr Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title_full_unstemmed Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title_short Rapid authenticity testing of artificially bred green turtles (Chelonia mydas) using microsatellite and mitochondrial DNA markers
title_sort rapid authenticity testing of artificially bred green turtles (chelonia mydas) using microsatellite and mitochondrial dna markers
topic Conservation Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557680/
https://www.ncbi.nlm.nih.gov/pubmed/34760392
http://dx.doi.org/10.7717/peerj.12410
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