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A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins

In this study, an IncI1 plasmid encoding resistance to both cefotaxime and azithromycin was recovered from a clinical Klebsiella pneumoniae strain. The azithromycin resistance was confirmed to be mediated by the erm(B) gene. This plasmid could be readily conjugated to strains of Escherichia coli and...

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Autores principales: Yang, Xuemei, Liu, Xiaoxuan, Yang, Chen, Chan, Edward Wai-Chi, Zhang, Rong, Chen, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557895/
https://www.ncbi.nlm.nih.gov/pubmed/34494862
http://dx.doi.org/10.1128/Spectrum.00286-21
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author Yang, Xuemei
Liu, Xiaoxuan
Yang, Chen
Chan, Edward Wai-Chi
Zhang, Rong
Chen, Sheng
author_facet Yang, Xuemei
Liu, Xiaoxuan
Yang, Chen
Chan, Edward Wai-Chi
Zhang, Rong
Chen, Sheng
author_sort Yang, Xuemei
collection PubMed
description In this study, an IncI1 plasmid encoding resistance to both cefotaxime and azithromycin was recovered from a clinical Klebsiella pneumoniae strain. The azithromycin resistance was confirmed to be mediated by the erm(B) gene. This plasmid could be readily conjugated to strains of Escherichia coli and Salmonella, promoting rapid dissemination of azithromycin- and ceftriaxone-resistance-encoding elements among Gram-negative bacterial pathogens. Transmission of this plasmid in Salmonella is of particular concern, since it could mediate expression of phenotypic resistance to azithromycin and ceftriaxone, which are the current choices for treatment of Salmonella infections. Our findings suggest a need to monitor the efficiency and pattern of transmission of this plasmid among key Gram-negative bacterial pathogens. IMPORTANCE Since the approval by the FDA of azithromycin for treatment of Salmonella infections, efforts have been made to monitor the development of resistance to azithromycin in these organisms. In this study, we report an IncI1 plasmid from a clinical K. pneumoniae strain that encodes resistance to both cefotaxime and azithromycin. This plasmid could be readily conjugated to strains of Escherichia coli and Salmonella, promoting rapid dissemination of azithromycin- and ceftriaxone-resistance-encoding elements among Gram-negative bacterial pathogens. Furthermore, data from this study confirmed for the first time the role of the erm(B) gene in mediating resistance to azithromycin in various bacterial species, particularly Salmonella.
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spelling pubmed-85578952021-11-08 A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins Yang, Xuemei Liu, Xiaoxuan Yang, Chen Chan, Edward Wai-Chi Zhang, Rong Chen, Sheng Microbiol Spectr Research Article In this study, an IncI1 plasmid encoding resistance to both cefotaxime and azithromycin was recovered from a clinical Klebsiella pneumoniae strain. The azithromycin resistance was confirmed to be mediated by the erm(B) gene. This plasmid could be readily conjugated to strains of Escherichia coli and Salmonella, promoting rapid dissemination of azithromycin- and ceftriaxone-resistance-encoding elements among Gram-negative bacterial pathogens. Transmission of this plasmid in Salmonella is of particular concern, since it could mediate expression of phenotypic resistance to azithromycin and ceftriaxone, which are the current choices for treatment of Salmonella infections. Our findings suggest a need to monitor the efficiency and pattern of transmission of this plasmid among key Gram-negative bacterial pathogens. IMPORTANCE Since the approval by the FDA of azithromycin for treatment of Salmonella infections, efforts have been made to monitor the development of resistance to azithromycin in these organisms. In this study, we report an IncI1 plasmid from a clinical K. pneumoniae strain that encodes resistance to both cefotaxime and azithromycin. This plasmid could be readily conjugated to strains of Escherichia coli and Salmonella, promoting rapid dissemination of azithromycin- and ceftriaxone-resistance-encoding elements among Gram-negative bacterial pathogens. Furthermore, data from this study confirmed for the first time the role of the erm(B) gene in mediating resistance to azithromycin in various bacterial species, particularly Salmonella. American Society for Microbiology 2021-09-08 /pmc/articles/PMC8557895/ /pubmed/34494862 http://dx.doi.org/10.1128/Spectrum.00286-21 Text en Copyright © 2021 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Yang, Xuemei
Liu, Xiaoxuan
Yang, Chen
Chan, Edward Wai-Chi
Zhang, Rong
Chen, Sheng
A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title_full A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title_fullStr A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title_full_unstemmed A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title_short A Conjugative IncI1 Plasmid Carrying erm(B) and bla(CTX-M-104) That Mediates Resistance to Azithromycin and Cephalosporins
title_sort conjugative inci1 plasmid carrying erm(b) and bla(ctx-m-104) that mediates resistance to azithromycin and cephalosporins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557895/
https://www.ncbi.nlm.nih.gov/pubmed/34494862
http://dx.doi.org/10.1128/Spectrum.00286-21
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