A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (gRNA) to t...

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Detalles Bibliográficos
Autores principales: Sachla, Ankita J., Alfonso, Alexander J., Helmann, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557940/
https://www.ncbi.nlm.nih.gov/pubmed/34523974
http://dx.doi.org/10.1128/Spectrum.00754-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557940/
https://www.ncbi.nlm.nih.gov/pubmed/34523974
http://dx.doi.org/10.1128/Spectrum.00754-21

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