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A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (gRNA) to t...

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Detalles Bibliográficos
Autores principales:	Sachla, Ankita J., Alfonso, Alexander J., Helmann, John D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557940/ https://www.ncbi.nlm.nih.gov/pubmed/34523974 http://dx.doi.org/10.1128/Spectrum.00754-21

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