Cargando…

A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (gRNA) to t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sachla, Ankita J., Alfonso, Alexander J., Helmann, John D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557940/ https://www.ncbi.nlm.nih.gov/pubmed/34523974 http://dx.doi.org/10.1128/Spectrum.00754-21

Ejemplares similares

Mutations of the Bacillus subtilis YidC1 (SpoIIIJ) insertase alleviate stress associated with σ(M)-dependent membrane protein overproduction
por: Zhao, Heng, et al.
Publicado: (2019)

CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis
por: Price, Marcus A., et al.
Publicado: (2019)

Engineering of Multiple Modules to Improve Amorphadiene Production in Bacillus subtilis Using CRISPR-Cas9
por: Song, Yafeng, et al.
Publicado: (2021)

A CRISPR-Cas9-Based Toolkit for Fast and Precise In Vivo Genetic Engineering of Bacillus subtilis Phages
por: Schilling, Tobias, et al.
Publicado: (2018)

A bacterial checkpoint protein for ribosome assembly moonlights as an essential metabolite-proofreading enzyme
por: Sachla, Ankita J., et al.
Publicado: (2019)

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis
por: So, Younju, et al.
Publicado: (2017)

TerC Proteins Function During Protein Secretion to Metalate Exoenzymes
por: He, Bixi, et al.
Publicado: (2023)

TerC proteins function during protein secretion to metalate exoenzymes
por: He, Bixi, et al.
Publicado: (2023)

TerC Proteins Function During Protein Secretion to Metalate Exoenzymes
por: He, Bixi, et al.
Publicado: (2023)

Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system
por: Zhang, Kang, et al.
Publicado: (2016)

Molecular logic of the Zur-regulated zinc deprivation response in Bacillus subtilis
por: Shin, Jung-Ho, et al.
Publicado: (2016)

Sequential binding and sensing of Zn(II) by Bacillus subtilis Zur
por: Ma, Zhen, et al.
Publicado: (2011)

A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering
por: Sebo, Zachary L, et al.
Publicado: (2014)

Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
por: Liu, Dingyu, et al.
Publicado: (2019)

Fragment Exchange Plasmid Tools for CRISPR/Cas9-Mediated Gene Integration and Protease Production in Bacillus subtilis
por: García-Moyano, Antonio, et al.
Publicado: (2020)

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination
por: Dalvie, Neil C., et al.
Publicado: (2021)

Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System
por: Wang, Yanchun, et al.
Publicado: (2019)

Programmed gRNA Removal System for CRISPR-Cas9-Mediated Multi-Round Genome Editing in Bacillus subtilis
por: Lim, Hayeon, et al.
Publicado: (2019)

Improvement of Bacilysin Production in Bacillus subtilis by CRISPR/Cas9-Mediated Editing of the 5’-Untranslated Region of the bac Operon
por: Abdulmalek, Hadeel Waleed, et al.
Publicado: (2023)

A simplified curcumin targets the membrane of Bacillus subtilis
por: Morão, Luana G., et al.
Publicado: (2018)

Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis
por: Zou, Yu, et al.
Publicado: (2022)

Intracellular Zn(II) Intoxication Leads to Dysregulation of the PerR Regulon Resulting in Heme Toxicity in Bacillus subtilis
por: Chandrangsu, Pete, et al.
Publicado: (2016)

Identification of altered function alleles that affect Bacillus subtilis PerR metal ion selectivity
por: Ma, Zhen, et al.
Publicado: (2011)

Iron homeostasis in Bacillus subtilis relies on three differentially expressed efflux systems
por: Steingard, Caroline H., et al.
Publicado: (2023)

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach
por: Seleit, Ali, et al.
Publicado: (2021)

Bacillus subtilis PgcA moonlights as a phosphoglucosamine mutase in support of peptidoglycan synthesis
por: Patel, Vaidehi, et al.
Publicado: (2019)

Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system
por: Watzlawick, Hildegard, et al.
Publicado: (2019)

Chromosome engineering in zygotes with CRISPR/Cas9
por: Boroviak, Katharina, et al.
Publicado: (2016)

Stabilization of Bacillus subtilis Spx under cell wall stress requires the anti-adaptor protein YirB
por: Rojas-Tapias, Daniel F., et al.
Publicado: (2018)

Genome-Wide Characterization of the Fur Regulatory Network Reveals a Link between Catechol Degradation and Bacillibactin Metabolism in Bacillus subtilis
por: Pi, Hualiang, et al.
Publicado: (2018)

CRISPR/Cas9-engineered Drosophila knock-in models to study VCP diseases
por: Wall, Jordan M., et al.
Publicado: (2021)

A metabolic checkpoint protein GlmR is important for diverting carbon into peptidoglycan biosynthesis in Bacillus subtilis
por: Patel, Vaidehi, et al.
Publicado: (2018)

Metabolic engineering of Escherichia coli BL21 strain using simplified CRISPR-Cas9 and asymmetric homology arms recombineering
por: Shukal, Sudha, et al.
Publicado: (2022)

Highly parallel genome variant engineering with CRISPR/Cas9
por: Sadhu, Meru J., et al.
Publicado: (2018)

Applications of CRISPR-Cas9 mediated genome engineering
por: Yang, Xiao
Publicado: (2015)

The Bacillus subtilis monothiol bacilliredoxin BrxC (YtxJ) and the Bdr (YpdA) disulfide reductase reduce S-bacillithiolated proteins
por: Gaballa, Ahmed, et al.
Publicado: (2021)

Protein Engineering Strategies to Expand CRISPR-Cas9 Applications
por: Ribeiro, Lucas F., et al.
Publicado: (2018)

Engineered CRISPR-Cas9 nucleases with altered PAM specificities
por: Kleinstiver, Benjamin P., et al.
Publicado: (2015)

Engineering large-scale chromosomal deletions by CRISPR-Cas9
por: Eleveld, Thomas F, et al.
Publicado: (2021)

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
por: Gratz, Scott J., et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_1rtpic6n37buou1pa5jb6j083f