Cargando…

Enhancement of Activity and Thermostability of Keratinase From Pseudomonas aeruginosa CCTCC AB2013184 by Directed Evolution With Noncanonical Amino Acids

A keratinase from Pseudomonas aeruginosa (KerPA), which belongs to the M4 family of metallopeptidases, was characterised in this study. This enzyme was engineered with non-canonical amino acids (ncAAs) using genetic code expansion. Several variants with enhanced activity and thermostability were ide...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pan, Xianchao, Yang, Jian, Xie, Peijuan, Zhang, Jing, Ke, Famin, Guo, Xiurong, Liang, Manyu, Liu, Li, Wang, Qin, Gao, Xiaowei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558439/ https://www.ncbi.nlm.nih.gov/pubmed/34733836 http://dx.doi.org/10.3389/fbioe.2021.770907

Ejemplares similares

Fusarium oxysporum and Aspergillus sp. as Keratinase Producers Using Swine Hair From Agroindustrial Residues
por: Preczeski, Karina Paula, et al.
Publicado: (2020)

Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR
por: Guan, Lijun, et al.
Publicado: (2020)

Efficient Selection Scheme for Incorporating Noncanonical Amino Acids Into Proteins in Saccharomyces cerevisiae
por: Tan, Linzhi, et al.
Publicado: (2020)

Corrigendum: Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR
por: Guan, Lijun, et al.
Publicado: (2020)

Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase
por: Yi, Huawei, et al.
Publicado: (2021)

Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
por: Espinoza-Vergara, Javier, et al.
Publicado: (2021)

Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa
por: Jabłońska, Joanna, et al.
Publicado: (2022)

Thermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization
por: Isupov, Michail N., et al.
Publicado: (2019)

Study of key amino acid residues of GH66 dextranase for producing high-degree polymerized isomaltooligosaccharides and improving of thermostability
por: Lin, Qianru, et al.
Publicado: (2022)

Pseudomonas aeruginosa RTE4: A Tea Rhizobacterium With Potential for Plant Growth Promotion and Biosurfactant Production
por: Chopra, Ankita, et al.
Publicado: (2020)

Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa
por: El-Deeb, Nehal M., et al.
Publicado: (2020)

Corrigendum: Thermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization
por: Isupov, Michail N., et al.
Publicado: (2019)

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective
por: Ajeje, Samaila Boyi, et al.
Publicado: (2021)

High Di-rhamnolipid Production Using Pseudomonas aeruginosa KT1115, Separation of Mono/Di-rhamnolipids, and Evaluation of Their Properties
por: Zhou, Jie, et al.
Publicado: (2019)

Targeted Genome Reduction of Pseudomonas aeruginosa Strain PAO1 Led to the Development of Hypovirulent and Hypersusceptible rDNA Hosts
por: Grosjean, Mélanie, et al.
Publicado: (2021)

Biochemical and Structural Properties of a Thermostable Mercuric Ion Reductase from Metallosphaera sedula
por: Artz, Jacob H., et al.
Publicado: (2015)

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13
por: Yadav, Punam, et al.
Publicado: (2018)

Purification and Characterization of Nit(phym), a Robust Thermostable Nitrilase From Paraburkholderia phymatum
por: Bessonnet, Thomas, et al.
Publicado: (2021)

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases
por: Nawaz, Ali, et al.
Publicado: (2023)

Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases
por: Chen, Zhongxiu, et al.
Publicado: (2022)

Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon
por: Leis, Benedikt, et al.
Publicado: (2015)

Expression, Characterization and Its Deinking Potential of a Thermostable Xylanase From Planomicrobium glaciei CHR43
por: Liu, Zhaoxing, et al.
Publicado: (2021)

A thermostable and CBM2-linked GH10 xylanase from Thermobifida fusca for paper bleaching
por: Wu, Xiuyun, et al.
Publicado: (2022)

An Insight Into Ameliorating Production, Catalytic Efficiency, Thermostability and Starch Saccharification of Acid-Stable α-Amylases From Acidophiles
por: Parashar, Deepak, et al.
Publicado: (2018)

Enhancing the Thermostability of Papain by Immobilizing on Deep Eutectic Solvents-Treated Chitosan With Optimal Microporous Structure and Catalytic Microenvironment
por: Lin, Kai-Peng, et al.
Publicado: (2020)

N-Ribosyltransferase From Archaeoglobus veneficus: A Novel Halotolerant and Thermostable Biocatalyst for the Synthesis of Purine Ribonucleoside Analogs
por: Acosta, Javier, et al.
Publicado: (2020)

Engineering Cofactor Specificity of a Thermostable Phosphite Dehydrogenase for a Highly Efficient and Robust NADPH Regeneration System
por: Abdel-Hady, Gamal Nasser, et al.
Publicado: (2021)

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis
por: Chen, Meiqi, et al.
Publicado: (2022)

Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
por: Zhang, Ning, et al.
Publicado: (2023)

Biochemical purification and characterization of a truncated acidic, thermostable chitinase from marine fungus for N-acetylglucosamine production
por: He, Bin, et al.
Publicado: (2022)

Highly Thermostable Xylanase Production from A Thermophilic Geobacillus sp. Strain WSUCF1 Utilizing Lignocellulosic Biomass
por: Bhalla, Aditya, et al.
Publicado: (2015)

Characterization and Secretory Expression of a Thermostable Tannase from Aureobasidium melanogenum T9: Potential Candidate for Food and Agricultural Industries
por: Liu, Lu, et al.
Publicado: (2022)

Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
por: Xing, Hongguan, et al.
Publicado: (2023)

Improved secretory expression and characterization of thermostable xylanase and β-xylosidase from Pseudothermotoga thermarum and their application in synergistic degradation of lignocellulose
por: Chen, Jinkang, et al.
Publicado: (2023)

Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides
por: Zhang, Xiu, et al.
Publicado: (2022)

Strategies for improved rhamnolipid production by Pseudomonas aeruginosa PA1
por: Soares dos Santos, Alexandre, et al.
Publicado: (2016)

The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
por: Koch, Moritz, et al.
Publicado: (2022)

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development
por: Fu, Jianmei, et al.
Publicado: (2020)

Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1
por: Zhou, Jie, et al.
Publicado: (2020)

Corrigendum: The survivor strain: Isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
por: Koch, Moritz, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_6u2iqe76clo3iu15lc83o1j4be