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Enhancement of Activity and Thermostability of Keratinase From Pseudomonas aeruginosa CCTCC AB2013184 by Directed Evolution With Noncanonical Amino Acids

A keratinase from Pseudomonas aeruginosa (KerPA), which belongs to the M4 family of metallopeptidases, was characterised in this study. This enzyme was engineered with non-canonical amino acids (ncAAs) using genetic code expansion. Several variants with enhanced activity and thermostability were ide...

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Detalles Bibliográficos
Autores principales:	Pan, Xianchao, Yang, Jian, Xie, Peijuan, Zhang, Jing, Ke, Famin, Guo, Xiurong, Liang, Manyu, Liu, Li, Wang, Qin, Gao, Xiaowei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558439/ https://www.ncbi.nlm.nih.gov/pubmed/34733836 http://dx.doi.org/10.3389/fbioe.2021.770907

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