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Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training

Training-induced follow-up of multiple muscle plasticity parameters in postural stability vs. locomotion muscles provides an integrative physiological view on shifts in the muscular metabolic machinery. It can be expected that not all muscle plasticity parameters show the same expression time profil...

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Autores principales: de Meeûs d'Argenteuil, Constance, Boshuizen, Berit, Vidal Moreno de Vega, Carmen, Leybaert, Luc, de Maré, Lorie, Goethals, Klara, De Spiegelaere, Ward, Oosterlinck, Maarten, Delesalle, Cathérine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558477/
https://www.ncbi.nlm.nih.gov/pubmed/34733900
http://dx.doi.org/10.3389/fvets.2021.718866
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author de Meeûs d'Argenteuil, Constance
Boshuizen, Berit
Vidal Moreno de Vega, Carmen
Leybaert, Luc
de Maré, Lorie
Goethals, Klara
De Spiegelaere, Ward
Oosterlinck, Maarten
Delesalle, Cathérine
author_facet de Meeûs d'Argenteuil, Constance
Boshuizen, Berit
Vidal Moreno de Vega, Carmen
Leybaert, Luc
de Maré, Lorie
Goethals, Klara
De Spiegelaere, Ward
Oosterlinck, Maarten
Delesalle, Cathérine
author_sort de Meeûs d'Argenteuil, Constance
collection PubMed
description Training-induced follow-up of multiple muscle plasticity parameters in postural stability vs. locomotion muscles provides an integrative physiological view on shifts in the muscular metabolic machinery. It can be expected that not all muscle plasticity parameters show the same expression time profile across muscles. This knowledge is important to underpin results of metabolomic studies. Twelve non-competing Standardbred mares were subjected to standardized harness training. Muscle biopsies were taken on a non-training day before and after 8 weeks. Shifts in muscle fiber type composition and muscle fiber cross-sectional area (CSA) were compared in the m. pectoralis, the m. vastus lateralis, and the m. semitendinosus. In the m. vastus lateralis, which showed most pronounced training-induced plasticity, two additional muscle plasticity parameters (capillarization and mitochondrial density) were assessed. In the m. semitendinosus, additionally the mean minimum Feret's diameter was assessed. There was a significant difference in baseline profiles. The m. semitendinosus contained less type I and more type IIX fibers compatible with the most pronounced anaerobic profile. Though no baseline fiber type-specific and overall mean CSA differences could be detected, there was a clear post-training decrease in fiber type specific CSA, most pronounced for the m. vastus lateralis, and this was accompanied by a clear increase in capillary supply. No shifts in mitochondrial density were detected. The m. semitendinosus showed a decrease in fiber type specific CSA of type IIAX fibers and a decrease of type I fiber Feret's diameter as well as mean minimum Feret's diameter. The training-induced increased capillary supply in conjunction with a significant decrease in muscle fiber CSA suggests that the muscular machinery models itself toward an optimal smaller individual muscle fiber structure to receive and process fuels that can be swiftly delivered by the circulatory system. These results are interesting in view of the recently identified important fuel candidates such as branched-chain amino acids, aromatic amino acids, and gut microbiome-related xenobiotics, which need a rapid gut–muscle gateway to reach these fibers and are less challenging for the mitochondrial system. More research is needed with that respect. Results also show important differences between muscle groups with respect to baseline and training-specific modulation.
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spelling pubmed-85584772021-11-02 Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training de Meeûs d'Argenteuil, Constance Boshuizen, Berit Vidal Moreno de Vega, Carmen Leybaert, Luc de Maré, Lorie Goethals, Klara De Spiegelaere, Ward Oosterlinck, Maarten Delesalle, Cathérine Front Vet Sci Veterinary Science Training-induced follow-up of multiple muscle plasticity parameters in postural stability vs. locomotion muscles provides an integrative physiological view on shifts in the muscular metabolic machinery. It can be expected that not all muscle plasticity parameters show the same expression time profile across muscles. This knowledge is important to underpin results of metabolomic studies. Twelve non-competing Standardbred mares were subjected to standardized harness training. Muscle biopsies were taken on a non-training day before and after 8 weeks. Shifts in muscle fiber type composition and muscle fiber cross-sectional area (CSA) were compared in the m. pectoralis, the m. vastus lateralis, and the m. semitendinosus. In the m. vastus lateralis, which showed most pronounced training-induced plasticity, two additional muscle plasticity parameters (capillarization and mitochondrial density) were assessed. In the m. semitendinosus, additionally the mean minimum Feret's diameter was assessed. There was a significant difference in baseline profiles. The m. semitendinosus contained less type I and more type IIX fibers compatible with the most pronounced anaerobic profile. Though no baseline fiber type-specific and overall mean CSA differences could be detected, there was a clear post-training decrease in fiber type specific CSA, most pronounced for the m. vastus lateralis, and this was accompanied by a clear increase in capillary supply. No shifts in mitochondrial density were detected. The m. semitendinosus showed a decrease in fiber type specific CSA of type IIAX fibers and a decrease of type I fiber Feret's diameter as well as mean minimum Feret's diameter. The training-induced increased capillary supply in conjunction with a significant decrease in muscle fiber CSA suggests that the muscular machinery models itself toward an optimal smaller individual muscle fiber structure to receive and process fuels that can be swiftly delivered by the circulatory system. These results are interesting in view of the recently identified important fuel candidates such as branched-chain amino acids, aromatic amino acids, and gut microbiome-related xenobiotics, which need a rapid gut–muscle gateway to reach these fibers and are less challenging for the mitochondrial system. More research is needed with that respect. Results also show important differences between muscle groups with respect to baseline and training-specific modulation. Frontiers Media S.A. 2021-10-18 /pmc/articles/PMC8558477/ /pubmed/34733900 http://dx.doi.org/10.3389/fvets.2021.718866 Text en Copyright © 2021 de Meeûs d'Argenteuil, Boshuizen, Vidal Moreno de Vega, Leybaert, de Maré, Goethals, De Spiegelaere, Oosterlinck and Delesalle. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
de Meeûs d'Argenteuil, Constance
Boshuizen, Berit
Vidal Moreno de Vega, Carmen
Leybaert, Luc
de Maré, Lorie
Goethals, Klara
De Spiegelaere, Ward
Oosterlinck, Maarten
Delesalle, Cathérine
Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title_full Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title_fullStr Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title_full_unstemmed Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title_short Comparison of Shifts in Skeletal Muscle Plasticity Parameters in Horses in Three Different Muscles, in Answer to 8 Weeks of Harness Training
title_sort comparison of shifts in skeletal muscle plasticity parameters in horses in three different muscles, in answer to 8 weeks of harness training
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558477/
https://www.ncbi.nlm.nih.gov/pubmed/34733900
http://dx.doi.org/10.3389/fvets.2021.718866
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