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Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions
Almost all cervical cancer is associated with persistent infection of high-risk (hr) human papillomavirus (HPV) like HPV16. The E7 oncoprotein encoded by hrHPV plays a crucial role in carcinogenesis. The present study aimed to establish a reliable protocol of immunohistochemistry stains to detect HP...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558648/ https://www.ncbi.nlm.nih.gov/pubmed/34729114 http://dx.doi.org/10.7150/jca.60554 |
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author | Zhu, Jiang Chen, Lingjing Zheng, Qiaoli Han, Rui Chen, Xianzhen Zhou, Qiang Cheng, Hao |
author_facet | Zhu, Jiang Chen, Lingjing Zheng, Qiaoli Han, Rui Chen, Xianzhen Zhou, Qiang Cheng, Hao |
author_sort | Zhu, Jiang |
collection | PubMed |
description | Almost all cervical cancer is associated with persistent infection of high-risk (hr) human papillomavirus (HPV) like HPV16. The E7 oncoprotein encoded by hrHPV plays a crucial role in carcinogenesis. The present study aimed to establish a reliable protocol of immunohistochemistry stains to detect HPV16 E7 protein in formalin-fixed and paraffin-embedded tissue specimens of various cervical lesions. Firstly, the HPV16 E7 gene was inserted into a prokaryotic expression vector pGEX-4T2. Then the recombinant plasmid pGEX-4T2-(HPV16-E7) was transformed into Escherichia coli JM109. The fusion protein containing a GST tag was purified, and New Zealand white rabbits were immunized to produce the HPV16 E7 polyclonal antibody. The anti-HPV16 E7 antibody was verified by western blotting and immunofluorescence stains, and applied in 182 HPV16 DNA-positive cervical tissue specimens and matched 36 HPV DNA-negative specimens by immunohistochemistry. Furthermore, A positive correlation between HPV16 E7 protein expression and malignancy grade was observed. But there is no relationship between HPV16 E7 protein expression and tumor sizes, tumor differentiation, lymph node metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage, or lymphovascular space invasion in cervical cancer. These findings provide a basis for further research focusing on the role of HPV E7 protein in various HPV-related diseases. |
format | Online Article Text |
id | pubmed-8558648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-85586482021-11-01 Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions Zhu, Jiang Chen, Lingjing Zheng, Qiaoli Han, Rui Chen, Xianzhen Zhou, Qiang Cheng, Hao J Cancer Research Paper Almost all cervical cancer is associated with persistent infection of high-risk (hr) human papillomavirus (HPV) like HPV16. The E7 oncoprotein encoded by hrHPV plays a crucial role in carcinogenesis. The present study aimed to establish a reliable protocol of immunohistochemistry stains to detect HPV16 E7 protein in formalin-fixed and paraffin-embedded tissue specimens of various cervical lesions. Firstly, the HPV16 E7 gene was inserted into a prokaryotic expression vector pGEX-4T2. Then the recombinant plasmid pGEX-4T2-(HPV16-E7) was transformed into Escherichia coli JM109. The fusion protein containing a GST tag was purified, and New Zealand white rabbits were immunized to produce the HPV16 E7 polyclonal antibody. The anti-HPV16 E7 antibody was verified by western blotting and immunofluorescence stains, and applied in 182 HPV16 DNA-positive cervical tissue specimens and matched 36 HPV DNA-negative specimens by immunohistochemistry. Furthermore, A positive correlation between HPV16 E7 protein expression and malignancy grade was observed. But there is no relationship between HPV16 E7 protein expression and tumor sizes, tumor differentiation, lymph node metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage, or lymphovascular space invasion in cervical cancer. These findings provide a basis for further research focusing on the role of HPV E7 protein in various HPV-related diseases. Ivyspring International Publisher 2021-10-20 /pmc/articles/PMC8558648/ /pubmed/34729114 http://dx.doi.org/10.7150/jca.60554 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhu, Jiang Chen, Lingjing Zheng, Qiaoli Han, Rui Chen, Xianzhen Zhou, Qiang Cheng, Hao Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title | Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title_full | Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title_fullStr | Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title_full_unstemmed | Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title_short | Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions |
title_sort | immunohistochemical detection of human papillomavirus 16 e7 oncoprotein in cervical lesions |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558648/ https://www.ncbi.nlm.nih.gov/pubmed/34729114 http://dx.doi.org/10.7150/jca.60554 |
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