Cargando…
Kinetic sequencing (k-Seq) as a massively parallel assay for ribozyme kinetics: utility and critical parameters
Characterizing genotype-phenotype relationships of biomolecules (e.g. ribozymes) requires accurate ways to measure activity for a large set of molecules. Kinetic measurement using high-throughput sequencing (e.g. k-Seq) is an emerging assay applicable in various domains that potentially scales up me...
Autores principales: | Shen, Yuning, Pressman, Abe, Janzen, Evan, Chen, Irene A |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559535/ https://www.ncbi.nlm.nih.gov/pubmed/33772580 http://dx.doi.org/10.1093/nar/gkab199 |
Ejemplares similares
-
High-throughput assay and engineering of self-cleaving ribozymes by sequencing
por: Kobori, Shungo, et al.
Publicado: (2015) -
Absolute quantitative measurement of transcriptional kinetic parameters in vivo
por: Iyer, Sukanya, et al.
Publicado: (2016) -
Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay
por: Ausländer, Simon, et al.
Publicado: (2016) -
A combinatorial method to isolate short ribozymes from complex ribozyme libraries
por: Arriola, Joshua T, et al.
Publicado: (2020) -
Bind-n-Seq: high-throughput analysis of in vitro protein–DNA interactions using massively parallel sequencing
por: Zykovich, Artem, et al.
Publicado: (2009)