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Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis
Osteoarthritis (OA) is a degenerative disorder that can result in the loss of articular cartilage. No effective treatment against OA is currently available. Thus, interest in natural health products to relieve OA symptoms is increasing. However, their qualities such as efficacy, toxicity, and mechan...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560463/ https://www.ncbi.nlm.nih.gov/pubmed/34488936 http://dx.doi.org/10.5483/BMBRep.2021.54.10.108 |
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author | Tran, Thanh-Tam Song, Won-Hyun Lee, Gyuseok Kim, Hyung Seok Park, Daeho Huh, Yun Hyun Ryu, Je-Hwang |
author_facet | Tran, Thanh-Tam Song, Won-Hyun Lee, Gyuseok Kim, Hyung Seok Park, Daeho Huh, Yun Hyun Ryu, Je-Hwang |
author_sort | Tran, Thanh-Tam |
collection | PubMed |
description | Osteoarthritis (OA) is a degenerative disorder that can result in the loss of articular cartilage. No effective treatment against OA is currently available. Thus, interest in natural health products to relieve OA symptoms is increasing. However, their qualities such as efficacy, toxicity, and mechanism are poorly understood. In this study, we determined the efficacy of avenanthramide (Avn)-C extracted from oats as a promising candidate to prevent OA progression and its mechanism of action to prevent the expression of matrix-metalloproteinases (MMPs) in OA pathogenesis. Interleukin-1 beta (IL-1β), a proinflammatory cytokine as a main causing factor of cartilage destruction, was used to induce OA-like condition of chondrocytes in vitro. Avn-C restrained IL-1β-mediated expression and activity of MMPs, such as MMP-3, -12, and -13 in mouse articular chondrocytes. Moreover, Avn-C alleviated cartilage destruction in experimental OA mouse model induced by destabilization of the medial meniscus (DMM) surgery. However, Avn-C did not affect the expression of inflammatory mediators (Ptgs2 and Nos) or anabolic factors (Col2a1, Aggrecan, and Sox9), although expression levels of these genes were upregulated or downregulated by IL-1β, respectively. The inhibition of MMP expression by Avn-C in articular chondrocytes was mediated by p38 kinase and c-Jun N-terminal kinase (JNK) signaling, but not by ERK or NF-κB. Interestingly, Avn-C added with SB203580 and SP600125 as specific inhibitors of p38 kinase and JNK, respectively, enhanced its inhibitory effect on the expression of MMPs in IL-1β treated chondrocytes. Taken together, these results suggest that Avn-C is an effective candidate to prevent OA progression and a natural health product to relieve OA pathogenesis. |
format | Online Article Text |
id | pubmed-8560463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-85604632021-11-12 Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis Tran, Thanh-Tam Song, Won-Hyun Lee, Gyuseok Kim, Hyung Seok Park, Daeho Huh, Yun Hyun Ryu, Je-Hwang BMB Rep Article Osteoarthritis (OA) is a degenerative disorder that can result in the loss of articular cartilage. No effective treatment against OA is currently available. Thus, interest in natural health products to relieve OA symptoms is increasing. However, their qualities such as efficacy, toxicity, and mechanism are poorly understood. In this study, we determined the efficacy of avenanthramide (Avn)-C extracted from oats as a promising candidate to prevent OA progression and its mechanism of action to prevent the expression of matrix-metalloproteinases (MMPs) in OA pathogenesis. Interleukin-1 beta (IL-1β), a proinflammatory cytokine as a main causing factor of cartilage destruction, was used to induce OA-like condition of chondrocytes in vitro. Avn-C restrained IL-1β-mediated expression and activity of MMPs, such as MMP-3, -12, and -13 in mouse articular chondrocytes. Moreover, Avn-C alleviated cartilage destruction in experimental OA mouse model induced by destabilization of the medial meniscus (DMM) surgery. However, Avn-C did not affect the expression of inflammatory mediators (Ptgs2 and Nos) or anabolic factors (Col2a1, Aggrecan, and Sox9), although expression levels of these genes were upregulated or downregulated by IL-1β, respectively. The inhibition of MMP expression by Avn-C in articular chondrocytes was mediated by p38 kinase and c-Jun N-terminal kinase (JNK) signaling, but not by ERK or NF-κB. Interestingly, Avn-C added with SB203580 and SP600125 as specific inhibitors of p38 kinase and JNK, respectively, enhanced its inhibitory effect on the expression of MMPs in IL-1β treated chondrocytes. Taken together, these results suggest that Avn-C is an effective candidate to prevent OA progression and a natural health product to relieve OA pathogenesis. Korean Society for Biochemistry and Molecular Biology 2021-10-31 2021-10-31 /pmc/articles/PMC8560463/ /pubmed/34488936 http://dx.doi.org/10.5483/BMBRep.2021.54.10.108 Text en Copyright © 2021 by the The Korean Society for Biochemistry and Molecular Biology https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Tran, Thanh-Tam Song, Won-Hyun Lee, Gyuseok Kim, Hyung Seok Park, Daeho Huh, Yun Hyun Ryu, Je-Hwang Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title | Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title_full | Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title_fullStr | Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title_full_unstemmed | Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title_short | Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis |
title_sort | avenanthramide c as a novel candidate to alleviate osteoarthritic pathogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560463/ https://www.ncbi.nlm.nih.gov/pubmed/34488936 http://dx.doi.org/10.5483/BMBRep.2021.54.10.108 |
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