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Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5(T), was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560859/ https://www.ncbi.nlm.nih.gov/pubmed/34725408 http://dx.doi.org/10.1038/s41598-021-00865-8 |
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author | Saeedi, Ahmed Cummings, Nicola J. McLean, Denise Connerton, Ian F. Connerton, Phillippa L. |
author_facet | Saeedi, Ahmed Cummings, Nicola J. McLean, Denise Connerton, Ian F. Connerton, Phillippa L. |
author_sort | Saeedi, Ahmed |
collection | PubMed |
description | A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5(T), was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37 °C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16S rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5(T) confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5(T) share 16S rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5(T) represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5(T) as the type strain. |
format | Online Article Text |
id | pubmed-8560859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85608592021-11-03 Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator Saeedi, Ahmed Cummings, Nicola J. McLean, Denise Connerton, Ian F. Connerton, Phillippa L. Sci Rep Article A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5(T), was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37 °C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16S rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5(T) confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5(T) share 16S rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5(T) represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5(T) as the type strain. Nature Publishing Group UK 2021-11-01 /pmc/articles/PMC8560859/ /pubmed/34725408 http://dx.doi.org/10.1038/s41598-021-00865-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Saeedi, Ahmed Cummings, Nicola J. McLean, Denise Connerton, Ian F. Connerton, Phillippa L. Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title | Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title_full | Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title_fullStr | Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title_full_unstemmed | Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title_short | Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
title_sort | venatorbacter cucullus gen. nov sp. nov a novel bacterial predator |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560859/ https://www.ncbi.nlm.nih.gov/pubmed/34725408 http://dx.doi.org/10.1038/s41598-021-00865-8 |
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