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Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffrac...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562656/ https://www.ncbi.nlm.nih.gov/pubmed/34804550 http://dx.doi.org/10.1107/S205225252100868X |
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author | Tegze, Miklós Bortel, Gábor |
author_facet | Tegze, Miklós Bortel, Gábor |
author_sort | Tegze, Miklós |
collection | PubMed |
description | In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffraction patterns in reciprocal space and reconstructing the 3D intensity distribution. Here, two orientation methods are compared: the expansion maximization compression (EMC) algorithm and the correlation maximization (CM) algorithm. To investigate the efficiency, reliability and accuracy of the methods at various XFEL pulse fluences, simulated diffraction patterns of biological molecules are used. |
format | Online Article Text |
id | pubmed-8562656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-85626562021-11-18 Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments Tegze, Miklós Bortel, Gábor IUCrJ Research Papers In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffraction patterns in reciprocal space and reconstructing the 3D intensity distribution. Here, two orientation methods are compared: the expansion maximization compression (EMC) algorithm and the correlation maximization (CM) algorithm. To investigate the efficiency, reliability and accuracy of the methods at various XFEL pulse fluences, simulated diffraction patterns of biological molecules are used. International Union of Crystallography 2021-10-07 /pmc/articles/PMC8562656/ /pubmed/34804550 http://dx.doi.org/10.1107/S205225252100868X Text en © Tegze and Bortel 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Tegze, Miklós Bortel, Gábor Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title | Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title_full | Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title_fullStr | Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title_full_unstemmed | Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title_short | Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments |
title_sort | comparison of emc and cm methods for orienting diffraction images in single-particle imaging experiments |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562656/ https://www.ncbi.nlm.nih.gov/pubmed/34804550 http://dx.doi.org/10.1107/S205225252100868X |
work_keys_str_mv | AT tegzemiklos comparisonofemcandcmmethodsfororientingdiffractionimagesinsingleparticleimagingexperiments AT bortelgabor comparisonofemcandcmmethodsfororientingdiffractionimagesinsingleparticleimagingexperiments |