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Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments

In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffrac...

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Detalles Bibliográficos
Autores principales: Tegze, Miklós, Bortel, Gábor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562656/
https://www.ncbi.nlm.nih.gov/pubmed/34804550
http://dx.doi.org/10.1107/S205225252100868X
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author Tegze, Miklós
Bortel, Gábor
author_facet Tegze, Miklós
Bortel, Gábor
author_sort Tegze, Miklós
collection PubMed
description In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffraction patterns in reciprocal space and reconstructing the 3D intensity distribution. Here, two orientation methods are compared: the expansion maximization compression (EMC) algorithm and the correlation maximization (CM) algorithm. To investigate the efficiency, reliability and accuracy of the methods at various XFEL pulse fluences, simulated diffraction patterns of biological molecules are used.
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spelling pubmed-85626562021-11-18 Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments Tegze, Miklós Bortel, Gábor IUCrJ Research Papers In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations. The crucial step of the data processing of SPI is finding the orientations of the recorded diffraction patterns in reciprocal space and reconstructing the 3D intensity distribution. Here, two orientation methods are compared: the expansion maximization compression (EMC) algorithm and the correlation maximization (CM) algorithm. To investigate the efficiency, reliability and accuracy of the methods at various XFEL pulse fluences, simulated diffraction patterns of biological molecules are used. International Union of Crystallography 2021-10-07 /pmc/articles/PMC8562656/ /pubmed/34804550 http://dx.doi.org/10.1107/S205225252100868X Text en © Tegze and Bortel 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Tegze, Miklós
Bortel, Gábor
Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title_full Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title_fullStr Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title_full_unstemmed Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title_short Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments
title_sort comparison of emc and cm methods for orienting diffraction images in single-particle imaging experiments
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562656/
https://www.ncbi.nlm.nih.gov/pubmed/34804550
http://dx.doi.org/10.1107/S205225252100868X
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