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Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography

X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provi...

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Autores principales: Storm, Selina L. S., Axford, Danny, Owen, Robin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562668/
https://www.ncbi.nlm.nih.gov/pubmed/34804543
http://dx.doi.org/10.1107/S2052252521008423
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author Storm, Selina L. S.
Axford, Danny
Owen, Robin L.
author_facet Storm, Selina L. S.
Axford, Danny
Owen, Robin L.
author_sort Storm, Selina L. S.
collection PubMed
description X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provide an extension of crystal lifetime via an increase in diffraction efficiency. To date, however, hardware limitations have negated any possible gains. Through the first use of a cadmium telluride EIGER2 detector and a beamline optimized for high-energy data collection, it is shown that at higher energies fewer crystals will be required to obtain complete data, as the diffracted intensity per unit dose increases by a factor of more than two between 12.4 and 25 keV. Additionally, these higher energy data can provide more information, as shown by a systematic increase in the high-resolution cutoff of the data collected. Taken together, these gains point to a high-energy future for synchrotron-based macromolecular crystallography.
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spelling pubmed-85626682021-11-18 Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography Storm, Selina L. S. Axford, Danny Owen, Robin L. IUCrJ Research Papers X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provide an extension of crystal lifetime via an increase in diffraction efficiency. To date, however, hardware limitations have negated any possible gains. Through the first use of a cadmium telluride EIGER2 detector and a beamline optimized for high-energy data collection, it is shown that at higher energies fewer crystals will be required to obtain complete data, as the diffracted intensity per unit dose increases by a factor of more than two between 12.4 and 25 keV. Additionally, these higher energy data can provide more information, as shown by a systematic increase in the high-resolution cutoff of the data collected. Taken together, these gains point to a high-energy future for synchrotron-based macromolecular crystallography. International Union of Crystallography 2021-09-09 /pmc/articles/PMC8562668/ /pubmed/34804543 http://dx.doi.org/10.1107/S2052252521008423 Text en © Storm, Axford and Owen 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Storm, Selina L. S.
Axford, Danny
Owen, Robin L.
Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title_full Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title_fullStr Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title_full_unstemmed Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title_short Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
title_sort experimental evidence for the benefits of higher x-ray energies for macromolecular crystallography
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562668/
https://www.ncbi.nlm.nih.gov/pubmed/34804543
http://dx.doi.org/10.1107/S2052252521008423
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