New and simplified method for drug combination studies by checkerboard assay

To evaluate the effect of two combined antimicrobial drugs, one method currently in use is the checkerboard assay in a 96-well microplate, which gives a good in vivo • Preparation of the microorganism inoculum and three concentrations of antimicrobial drugs. • Easy dispensing of all reagents into th...

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Autores principales: Bellio, Pierangelo, Fagnani, Lorenza, Nazzicone, Lisaurora, Celenza, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563647/
https://www.ncbi.nlm.nih.gov/pubmed/34754811
http://dx.doi.org/10.1016/j.mex.2021.101543
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author Bellio, Pierangelo
Fagnani, Lorenza
Nazzicone, Lisaurora
Celenza, Giuseppe
author_facet Bellio, Pierangelo
Fagnani, Lorenza
Nazzicone, Lisaurora
Celenza, Giuseppe
author_sort Bellio, Pierangelo
collection PubMed
description To evaluate the effect of two combined antimicrobial drugs, one method currently in use is the checkerboard assay in a 96-well microplate, which gives a good in vivo • Preparation of the microorganism inoculum and three concentrations of antimicrobial drugs. • Easy dispensing of all reagents into the microplates with a multichannel pipette. • Evaluation of the microorganism optical density (OD) by a microplate reader, and calculation of growth percentage for each of the 77 combinations.
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spelling pubmed-85636472021-11-08 New and simplified method for drug combination studies by checkerboard assay Bellio, Pierangelo Fagnani, Lorenza Nazzicone, Lisaurora Celenza, Giuseppe MethodsX Method Article To evaluate the effect of two combined antimicrobial drugs, one method currently in use is the checkerboard assay in a 96-well microplate, which gives a good in vivo • Preparation of the microorganism inoculum and three concentrations of antimicrobial drugs. • Easy dispensing of all reagents into the microplates with a multichannel pipette. • Evaluation of the microorganism optical density (OD) by a microplate reader, and calculation of growth percentage for each of the 77 combinations. Elsevier 2021-10-11 /pmc/articles/PMC8563647/ /pubmed/34754811 http://dx.doi.org/10.1016/j.mex.2021.101543 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Method Article
Bellio, Pierangelo
Fagnani, Lorenza
Nazzicone, Lisaurora
Celenza, Giuseppe
New and simplified method for drug combination studies by checkerboard assay
title New and simplified method for drug combination studies by checkerboard assay
title_full New and simplified method for drug combination studies by checkerboard assay
title_fullStr New and simplified method for drug combination studies by checkerboard assay
title_full_unstemmed New and simplified method for drug combination studies by checkerboard assay
title_short New and simplified method for drug combination studies by checkerboard assay
title_sort new and simplified method for drug combination studies by checkerboard assay
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563647/
https://www.ncbi.nlm.nih.gov/pubmed/34754811
http://dx.doi.org/10.1016/j.mex.2021.101543
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AT celenzagiuseppe newandsimplifiedmethodfordrugcombinationstudiesbycheckerboardassay

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