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Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species

Melon (Cucumis melo) is one of the top 10 fruits in the world, and its production often suffers due to soil-borne diseases. Grafting is an effective way to solve this problem. However, graft incompatibility between scion and rootstock limits the application of melon grafting. In this study, the melo...

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Autores principales: Xiong, Mu, Liu, Changjin, Guo, Liping, Wang, Jin, Wu, Xiangshuai, Li, Ling, Bie, Zhilong, Huang, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563831/
https://www.ncbi.nlm.nih.gov/pubmed/34745194
http://dx.doi.org/10.3389/fpls.2021.762889
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author Xiong, Mu
Liu, Changjin
Guo, Liping
Wang, Jin
Wu, Xiangshuai
Li, Ling
Bie, Zhilong
Huang, Yuan
author_facet Xiong, Mu
Liu, Changjin
Guo, Liping
Wang, Jin
Wu, Xiangshuai
Li, Ling
Bie, Zhilong
Huang, Yuan
author_sort Xiong, Mu
collection PubMed
description Melon (Cucumis melo) is one of the top 10 fruits in the world, and its production often suffers due to soil-borne diseases. Grafting is an effective way to solve this problem. However, graft incompatibility between scion and rootstock limits the application of melon grafting. In this study, the melon was grafted onto eight Cucurbitaceae species (cucumber, pumpkin, melon, luffa, wax gourd, bottle gourd, bitter gourd, and watermelon), and graft compatibility evaluation and anatomical observation were conducted. Taking melon homo-grafted plants as control, melon grafted onto cucumber and pumpkin rootstocks was compatible, while melon grafted onto luffa, wax gourd, bottle gourd, bitter gourd, and watermelon rootstocks was incompatible based on the scion dry weight on day 42 after grafting. Meanwhile, we found that starch–iodine staining of scion stem base is an index to predict graft compatibility earlier, on day 14 after grafting. Further, microsection observations showed that there was more cell proliferation at graft junction of melon hetero-grafted combinations; vascular reconnection occurred in all graft combinations. However, excess callose deposited at graft junction resulted in the blockage of photosynthate transport, thus, leading to starch accumulation in scion stem base, and finally graft incompatibility. In addition, undegraded necrotic layer fragments were observed at graft junctions of melon grafted onto incompatible bitter gourd and watermelon rootstocks. The above results provide clues for the selection and breeding of compatible Cucurbitaceae rootstocks of melon and demonstrate that starch accumulation in scion base and callose deposition at graft junction is associated with melon graft compatibility.
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spelling pubmed-85638312021-11-04 Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species Xiong, Mu Liu, Changjin Guo, Liping Wang, Jin Wu, Xiangshuai Li, Ling Bie, Zhilong Huang, Yuan Front Plant Sci Plant Science Melon (Cucumis melo) is one of the top 10 fruits in the world, and its production often suffers due to soil-borne diseases. Grafting is an effective way to solve this problem. However, graft incompatibility between scion and rootstock limits the application of melon grafting. In this study, the melon was grafted onto eight Cucurbitaceae species (cucumber, pumpkin, melon, luffa, wax gourd, bottle gourd, bitter gourd, and watermelon), and graft compatibility evaluation and anatomical observation were conducted. Taking melon homo-grafted plants as control, melon grafted onto cucumber and pumpkin rootstocks was compatible, while melon grafted onto luffa, wax gourd, bottle gourd, bitter gourd, and watermelon rootstocks was incompatible based on the scion dry weight on day 42 after grafting. Meanwhile, we found that starch–iodine staining of scion stem base is an index to predict graft compatibility earlier, on day 14 after grafting. Further, microsection observations showed that there was more cell proliferation at graft junction of melon hetero-grafted combinations; vascular reconnection occurred in all graft combinations. However, excess callose deposited at graft junction resulted in the blockage of photosynthate transport, thus, leading to starch accumulation in scion stem base, and finally graft incompatibility. In addition, undegraded necrotic layer fragments were observed at graft junctions of melon grafted onto incompatible bitter gourd and watermelon rootstocks. The above results provide clues for the selection and breeding of compatible Cucurbitaceae rootstocks of melon and demonstrate that starch accumulation in scion base and callose deposition at graft junction is associated with melon graft compatibility. Frontiers Media S.A. 2021-10-20 /pmc/articles/PMC8563831/ /pubmed/34745194 http://dx.doi.org/10.3389/fpls.2021.762889 Text en Copyright © 2021 Xiong, Liu, Guo, Wang, Wu, Li, Bie and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xiong, Mu
Liu, Changjin
Guo, Liping
Wang, Jin
Wu, Xiangshuai
Li, Ling
Bie, Zhilong
Huang, Yuan
Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title_full Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title_fullStr Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title_full_unstemmed Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title_short Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species
title_sort compatibility evaluation and anatomical observation of melon grafted onto eight cucurbitaceae species
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563831/
https://www.ncbi.nlm.nih.gov/pubmed/34745194
http://dx.doi.org/10.3389/fpls.2021.762889
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