Cargando…
The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563897/ https://www.ncbi.nlm.nih.gov/pubmed/34727228 http://dx.doi.org/10.1186/s12284-021-00531-7 |
_version_ | 1784593502332518400 |
---|---|
author | Hu, Jihong Huang, Liyu Chen, Guanglong Liu, Hui Zhang, Yesheng Zhang, Ru Zhang, Shilai Liu, Jintao Hu, Qingyi Hu, Fengyi Wang, Wen Ding, Yi |
author_facet | Hu, Jihong Huang, Liyu Chen, Guanglong Liu, Hui Zhang, Yesheng Zhang, Ru Zhang, Shilai Liu, Jintao Hu, Qingyi Hu, Fengyi Wang, Wen Ding, Yi |
author_sort | Hu, Jihong |
collection | PubMed |
description | Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00531-7. |
format | Online Article Text |
id | pubmed-8563897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-85638972021-11-15 The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice Hu, Jihong Huang, Liyu Chen, Guanglong Liu, Hui Zhang, Yesheng Zhang, Ru Zhang, Shilai Liu, Jintao Hu, Qingyi Hu, Fengyi Wang, Wen Ding, Yi Rice (N Y) Original Article Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00531-7. Springer US 2021-11-02 /pmc/articles/PMC8563897/ /pubmed/34727228 http://dx.doi.org/10.1186/s12284-021-00531-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Hu, Jihong Huang, Liyu Chen, Guanglong Liu, Hui Zhang, Yesheng Zhang, Ru Zhang, Shilai Liu, Jintao Hu, Qingyi Hu, Fengyi Wang, Wen Ding, Yi The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title | The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title_full | The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title_fullStr | The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title_full_unstemmed | The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title_short | The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice |
title_sort | elite alleles of osspl4 regulate grain size and increase grain yield in rice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563897/ https://www.ncbi.nlm.nih.gov/pubmed/34727228 http://dx.doi.org/10.1186/s12284-021-00531-7 |
work_keys_str_mv | AT hujihong theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huangliyu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT chenguanglong theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liuhui theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangyesheng theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangru theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangshilai theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liujintao theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huqingyi theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT hufengyi theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT wangwen theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT dingyi theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT hujihong eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huangliyu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT chenguanglong eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liuhui eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangyesheng eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangru eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT zhangshilai eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liujintao eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huqingyi eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT hufengyi eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT wangwen eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT dingyi eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice |