Protocol for the assessment of mTOR activity in mouse primary hepatocytes

We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yiel...

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Detalles Bibliográficos
Autores principales: Plata-Gómez, Ana Belén, Crespo, María, de la Calle Arregui, Celia, de Prado-Rivas, Lucía, Sabio, Guadalupe, Efeyan, Alejo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564195/
https://www.ncbi.nlm.nih.gov/pubmed/34755120
http://dx.doi.org/10.1016/j.xpro.2021.100918
Descripción
Sumario:We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021).

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