Protocol for the assessment of mTOR activity in mouse primary hepatocytes

We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yiel...

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Autores principales: Plata-Gómez, Ana Belén, Crespo, María, de la Calle Arregui, Celia, de Prado-Rivas, Lucía, Sabio, Guadalupe, Efeyan, Alejo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564195/
https://www.ncbi.nlm.nih.gov/pubmed/34755120
http://dx.doi.org/10.1016/j.xpro.2021.100918
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author Plata-Gómez, Ana Belén
Crespo, María
de la Calle Arregui, Celia
de Prado-Rivas, Lucía
Sabio, Guadalupe
Efeyan, Alejo
author_facet Plata-Gómez, Ana Belén
Crespo, María
de la Calle Arregui, Celia
de Prado-Rivas, Lucía
Sabio, Guadalupe
Efeyan, Alejo
author_sort Plata-Gómez, Ana Belén
collection PubMed
description We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021).
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spelling pubmed-85641952021-11-08 Protocol for the assessment of mTOR activity in mouse primary hepatocytes Plata-Gómez, Ana Belén Crespo, María de la Calle Arregui, Celia de Prado-Rivas, Lucía Sabio, Guadalupe Efeyan, Alejo STAR Protoc Protocol We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021). Elsevier 2021-10-29 /pmc/articles/PMC8564195/ /pubmed/34755120 http://dx.doi.org/10.1016/j.xpro.2021.100918 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Plata-Gómez, Ana Belén
Crespo, María
de la Calle Arregui, Celia
de Prado-Rivas, Lucía
Sabio, Guadalupe
Efeyan, Alejo
Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title_full Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title_fullStr Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title_full_unstemmed Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title_short Protocol for the assessment of mTOR activity in mouse primary hepatocytes
title_sort protocol for the assessment of mtor activity in mouse primary hepatocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564195/
https://www.ncbi.nlm.nih.gov/pubmed/34755120
http://dx.doi.org/10.1016/j.xpro.2021.100918
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