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Protocol for the assessment of mTOR activity in mouse primary hepatocytes

We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yiel...

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Detalles Bibliográficos
Autores principales:	Plata-Gómez, Ana Belén, Crespo, María, de la Calle Arregui, Celia, de Prado-Rivas, Lucía, Sabio, Guadalupe, Efeyan, Alejo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564195/ https://www.ncbi.nlm.nih.gov/pubmed/34755120 http://dx.doi.org/10.1016/j.xpro.2021.100918

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