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AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy

Vascular calcification (VC) in macrovascular and peripheral blood vessels is one of the main factors leading to diabetes mellitus (DM) and death. Apart from the induction of vascular calcification, advanced glycation end products (AGEs) have also been reported to modulate autophagy and apoptosis in...

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Autores principales: He, Hu-Qiang, Qu, Yuan-Qing, Kwan Law, Betty Yuen, Qiu, Cong-ling, Han, Yu, Ricardo de Seabra Rodrigues Dias, Ivo, Liu, Yong, Zhang, Jie, Wu, An-Guo, Wu, Cheng-Wen, Fai Mok, Simon Wing, Cheng, Xin, He, Yan-Zheng, Wai Wong, Vincent Kam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564286/
https://www.ncbi.nlm.nih.gov/pubmed/34744705
http://dx.doi.org/10.3389/fphar.2021.692431
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author He, Hu-Qiang
Qu, Yuan-Qing
Kwan Law, Betty Yuen
Qiu, Cong-ling
Han, Yu
Ricardo de Seabra Rodrigues Dias, Ivo
Liu, Yong
Zhang, Jie
Wu, An-Guo
Wu, Cheng-Wen
Fai Mok, Simon Wing
Cheng, Xin
He, Yan-Zheng
Wai Wong, Vincent Kam
author_facet He, Hu-Qiang
Qu, Yuan-Qing
Kwan Law, Betty Yuen
Qiu, Cong-ling
Han, Yu
Ricardo de Seabra Rodrigues Dias, Ivo
Liu, Yong
Zhang, Jie
Wu, An-Guo
Wu, Cheng-Wen
Fai Mok, Simon Wing
Cheng, Xin
He, Yan-Zheng
Wai Wong, Vincent Kam
author_sort He, Hu-Qiang
collection PubMed
description Vascular calcification (VC) in macrovascular and peripheral blood vessels is one of the main factors leading to diabetes mellitus (DM) and death. Apart from the induction of vascular calcification, advanced glycation end products (AGEs) have also been reported to modulate autophagy and apoptosis in DM. Autophagy plays a role in maintaining the stabilization of the external and internal microenvironment. This process is vital for regulating arteriosclerosis. However, the internal mechanisms of this pathogenic process are still unclear. Besides, the relationship among autophagy, apoptosis, and calcification in HASMCs upon AGEs exposure has not been reported in detail. In this study, we established a calcification model of SMC through the intervention of AGEs. It was found that the calcification was upregulated in AGEs treated HASMCs when autophagy and apoptosis were activated. In the country, AGEs-activated calcification and apoptosis were suppressed in Atg7 knockout cells or pretreated with wortmannin (WM), an autophagy inhibitor. These results provide new insights to conduct further investigations on the potential clinical applications for autophagy inhibitors in the treatment of diabetes-related vascular calcification.
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spelling pubmed-85642862021-11-04 AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy He, Hu-Qiang Qu, Yuan-Qing Kwan Law, Betty Yuen Qiu, Cong-ling Han, Yu Ricardo de Seabra Rodrigues Dias, Ivo Liu, Yong Zhang, Jie Wu, An-Guo Wu, Cheng-Wen Fai Mok, Simon Wing Cheng, Xin He, Yan-Zheng Wai Wong, Vincent Kam Front Pharmacol Pharmacology Vascular calcification (VC) in macrovascular and peripheral blood vessels is one of the main factors leading to diabetes mellitus (DM) and death. Apart from the induction of vascular calcification, advanced glycation end products (AGEs) have also been reported to modulate autophagy and apoptosis in DM. Autophagy plays a role in maintaining the stabilization of the external and internal microenvironment. This process is vital for regulating arteriosclerosis. However, the internal mechanisms of this pathogenic process are still unclear. Besides, the relationship among autophagy, apoptosis, and calcification in HASMCs upon AGEs exposure has not been reported in detail. In this study, we established a calcification model of SMC through the intervention of AGEs. It was found that the calcification was upregulated in AGEs treated HASMCs when autophagy and apoptosis were activated. In the country, AGEs-activated calcification and apoptosis were suppressed in Atg7 knockout cells or pretreated with wortmannin (WM), an autophagy inhibitor. These results provide new insights to conduct further investigations on the potential clinical applications for autophagy inhibitors in the treatment of diabetes-related vascular calcification. Frontiers Media S.A. 2021-10-20 /pmc/articles/PMC8564286/ /pubmed/34744705 http://dx.doi.org/10.3389/fphar.2021.692431 Text en Copyright © 2021 He, Qu, Kwan Law, Qiu, Han, Ricardo de Seabra Rodrigues Dias, Liu, Zhang, Wu, Wu, Fai Mok, Cheng, He and Wai Wong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
He, Hu-Qiang
Qu, Yuan-Qing
Kwan Law, Betty Yuen
Qiu, Cong-ling
Han, Yu
Ricardo de Seabra Rodrigues Dias, Ivo
Liu, Yong
Zhang, Jie
Wu, An-Guo
Wu, Cheng-Wen
Fai Mok, Simon Wing
Cheng, Xin
He, Yan-Zheng
Wai Wong, Vincent Kam
AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title_full AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title_fullStr AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title_full_unstemmed AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title_short AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy
title_sort ages-induced calcification and apoptosis in human vascular smooth muscle cells is reversed by inhibition of autophagy
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564286/
https://www.ncbi.nlm.nih.gov/pubmed/34744705
http://dx.doi.org/10.3389/fphar.2021.692431
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