Cargando…

Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota

Short-chain fatty acids (SCFAs) are metabolic products produced during the microbial fermentation of non-digestible fibers and play an important role in metabolic homeostasis and overall gut health. In this study, we investigated the effects of supplementation with multispecies probiotics (MSPs) con...

Descripción completa

Detalles Bibliográficos
Autores principales: Oh, Ju Kyoung, Vasquez, Robie, Kim, Sang Hoon, Hwang, In-Chan, Song, Ji Hoon, Park, Jae Hong, Kim, In Ho, Kang, Dae-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Animal Sciences and Technology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564300/
https://www.ncbi.nlm.nih.gov/pubmed/34796353
http://dx.doi.org/10.5187/jast.2021.e94
_version_ 1784593587433897984
author Oh, Ju Kyoung
Vasquez, Robie
Kim, Sang Hoon
Hwang, In-Chan
Song, Ji Hoon
Park, Jae Hong
Kim, In Ho
Kang, Dae-Kyung
author_facet Oh, Ju Kyoung
Vasquez, Robie
Kim, Sang Hoon
Hwang, In-Chan
Song, Ji Hoon
Park, Jae Hong
Kim, In Ho
Kang, Dae-Kyung
author_sort Oh, Ju Kyoung
collection PubMed
description Short-chain fatty acids (SCFAs) are metabolic products produced during the microbial fermentation of non-digestible fibers and play an important role in metabolic homeostasis and overall gut health. In this study, we investigated the effects of supplementation with multispecies probiotics (MSPs) containing Bacillus amyloliquefaciens, Limosilactobacillus reuteri, and Levilactobacillus brevis on the gut microbiota, and fecal SCFAs and lactate levels of weaned pigs. A total of 38 pigs weaned at 4 weeks of age were fed either a basal diet or a diet supplemented with MSPs for 6 weeks. MSP administration significantly increased the fecal concentrations of lactate (2.3-fold; p < 0.01), acetate (1.8-fold; p < 0.05), and formate (1.4-fold; p < 0.05). Moreover, MSP supplementation altered the gut microbiota of the pigs by significantly increasing the population of potentially beneficial bacteria such as Olsenella, Catonella, Catenibacterium, Acidaminococcus, and Ruminococcaceae. MSP supplementation also decreased the abundance of pathogenic bacteria such as Escherichia and Chlamydia. The modulation of the gut microbiota was observed to be strongly correlated with the changes in fecal SCFAs and lactate levels. Furthermore, we found changes in the functional pathways present within the gut, which supports our findings that MSP modulates the gut microbiota and SCFAs levels in pigs. The results support the potential use of MSPs to improve the gut health of animals by modulating SCFAs production.
format Online
Article
Text
id pubmed-8564300
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Korean Society of Animal Sciences and Technology
record_format MEDLINE/PubMed
spelling pubmed-85643002021-11-17 Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota Oh, Ju Kyoung Vasquez, Robie Kim, Sang Hoon Hwang, In-Chan Song, Ji Hoon Park, Jae Hong Kim, In Ho Kang, Dae-Kyung J Anim Sci Technol Research Article Short-chain fatty acids (SCFAs) are metabolic products produced during the microbial fermentation of non-digestible fibers and play an important role in metabolic homeostasis and overall gut health. In this study, we investigated the effects of supplementation with multispecies probiotics (MSPs) containing Bacillus amyloliquefaciens, Limosilactobacillus reuteri, and Levilactobacillus brevis on the gut microbiota, and fecal SCFAs and lactate levels of weaned pigs. A total of 38 pigs weaned at 4 weeks of age were fed either a basal diet or a diet supplemented with MSPs for 6 weeks. MSP administration significantly increased the fecal concentrations of lactate (2.3-fold; p < 0.01), acetate (1.8-fold; p < 0.05), and formate (1.4-fold; p < 0.05). Moreover, MSP supplementation altered the gut microbiota of the pigs by significantly increasing the population of potentially beneficial bacteria such as Olsenella, Catonella, Catenibacterium, Acidaminococcus, and Ruminococcaceae. MSP supplementation also decreased the abundance of pathogenic bacteria such as Escherichia and Chlamydia. The modulation of the gut microbiota was observed to be strongly correlated with the changes in fecal SCFAs and lactate levels. Furthermore, we found changes in the functional pathways present within the gut, which supports our findings that MSP modulates the gut microbiota and SCFAs levels in pigs. The results support the potential use of MSPs to improve the gut health of animals by modulating SCFAs production. Korean Society of Animal Sciences and Technology 2021-09 2021-09-30 /pmc/articles/PMC8564300/ /pubmed/34796353 http://dx.doi.org/10.5187/jast.2021.e94 Text en © Copyright 2021 Korean Society of Animal Science and Technology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Oh, Ju Kyoung
Vasquez, Robie
Kim, Sang Hoon
Hwang, In-Chan
Song, Ji Hoon
Park, Jae Hong
Kim, In Ho
Kang, Dae-Kyung
Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title_full Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title_fullStr Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title_full_unstemmed Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title_short Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
title_sort multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564300/
https://www.ncbi.nlm.nih.gov/pubmed/34796353
http://dx.doi.org/10.5187/jast.2021.e94
work_keys_str_mv AT ohjukyoung multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT vasquezrobie multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT kimsanghoon multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT hwanginchan multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT songjihoon multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT parkjaehong multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT kiminho multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota
AT kangdaekyung multispeciesprobioticsalterfecalshortchainfattyacidsandlactatelevelsinweanedpigsbymodulatinggutmicrobiota