Cargando…

Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection

The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Mingyue, Zheng, Mengjia, Wiraja, Christian, Chew, Sharon Wan Ting, Mishra, Arti, Mayandi, Venkatesh, Lakshminarayanan, Rajamani, Xu, Chenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564459/
https://www.ncbi.nlm.nih.gov/pubmed/34494724
http://dx.doi.org/10.1002/advs.202102327
_version_ 1784593622801317888
author Cui, Mingyue
Zheng, Mengjia
Wiraja, Christian
Chew, Sharon Wan Ting
Mishra, Arti
Mayandi, Venkatesh
Lakshminarayanan, Rajamani
Xu, Chenjie
author_facet Cui, Mingyue
Zheng, Mengjia
Wiraja, Christian
Chew, Sharon Wan Ting
Mishra, Arti
Mayandi, Venkatesh
Lakshminarayanan, Rajamani
Xu, Chenjie
author_sort Cui, Mingyue
collection PubMed
description The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long‐term storage studies, and they successfully impede the growth of gram‐negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders.
format Online
Article
Text
id pubmed-8564459
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-85644592021-11-09 Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie Adv Sci (Weinh) Research Articles The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long‐term storage studies, and they successfully impede the growth of gram‐negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. John Wiley and Sons Inc. 2021-09-08 /pmc/articles/PMC8564459/ /pubmed/34494724 http://dx.doi.org/10.1002/advs.202102327 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Cui, Mingyue
Zheng, Mengjia
Wiraja, Christian
Chew, Sharon Wan Ting
Mishra, Arti
Mayandi, Venkatesh
Lakshminarayanan, Rajamani
Xu, Chenjie
Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title_full Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title_fullStr Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title_full_unstemmed Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title_short Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
title_sort ocular delivery of predatory bacteria with cryomicroneedles against eye infection
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564459/
https://www.ncbi.nlm.nih.gov/pubmed/34494724
http://dx.doi.org/10.1002/advs.202102327
work_keys_str_mv AT cuimingyue oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT zhengmengjia oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT wirajachristian oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT chewsharonwanting oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT mishraarti oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT mayandivenkatesh oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT lakshminarayananrajamani oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection
AT xuchenjie oculardeliveryofpredatorybacteriawithcryomicroneedlesagainsteyeinfection