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Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection
The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564459/ https://www.ncbi.nlm.nih.gov/pubmed/34494724 http://dx.doi.org/10.1002/advs.202102327 |
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author | Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie |
author_facet | Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie |
author_sort | Cui, Mingyue |
collection | PubMed |
description | The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long‐term storage studies, and they successfully impede the growth of gram‐negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. |
format | Online Article Text |
id | pubmed-8564459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85644592021-11-09 Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie Adv Sci (Weinh) Research Articles The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long‐term storage studies, and they successfully impede the growth of gram‐negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. John Wiley and Sons Inc. 2021-09-08 /pmc/articles/PMC8564459/ /pubmed/34494724 http://dx.doi.org/10.1002/advs.202102327 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title | Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title_full | Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title_fullStr | Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title_full_unstemmed | Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title_short | Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection |
title_sort | ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564459/ https://www.ncbi.nlm.nih.gov/pubmed/34494724 http://dx.doi.org/10.1002/advs.202102327 |
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