An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-ad...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564727/ https://www.ncbi.nlm.nih.gov/pubmed/34754792 http://dx.doi.org/10.1016/j.mex.2021.101521 |
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author | Ali, Rizwan El-Boubbou, Kheireddine Boudjelal, Mohamed |
author_facet | Ali, Rizwan El-Boubbou, Kheireddine Boudjelal, Mohamed |
author_sort | Ali, Rizwan |
collection | PubMed |
description | Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging. • An easy and rapid method to visualize biological specimens under SEM. • Cells are grown on carbon tapes and gold coated. • Air drying without compromising the image quality. |
format | Online Article Text |
id | pubmed-8564727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85647272021-11-08 An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) Ali, Rizwan El-Boubbou, Kheireddine Boudjelal, Mohamed MethodsX Method Article Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging. • An easy and rapid method to visualize biological specimens under SEM. • Cells are grown on carbon tapes and gold coated. • Air drying without compromising the image quality. Elsevier 2021-09-20 /pmc/articles/PMC8564727/ /pubmed/34754792 http://dx.doi.org/10.1016/j.mex.2021.101521 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Method Article Ali, Rizwan El-Boubbou, Kheireddine Boudjelal, Mohamed An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title | An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title_full | An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title_fullStr | An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title_full_unstemmed | An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title_short | An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) |
title_sort | easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (sem) |
topic | Method Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564727/ https://www.ncbi.nlm.nih.gov/pubmed/34754792 http://dx.doi.org/10.1016/j.mex.2021.101521 |
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