An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)

Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-ad...

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Detalles Bibliográficos
Autores principales: Ali, Rizwan, El-Boubbou, Kheireddine, Boudjelal, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564727/
https://www.ncbi.nlm.nih.gov/pubmed/34754792
http://dx.doi.org/10.1016/j.mex.2021.101521
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author Ali, Rizwan
El-Boubbou, Kheireddine
Boudjelal, Mohamed
author_facet Ali, Rizwan
El-Boubbou, Kheireddine
Boudjelal, Mohamed
author_sort Ali, Rizwan
collection PubMed
description Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging. • An easy and rapid method to visualize biological specimens under SEM. • Cells are grown on carbon tapes and gold coated. • Air drying without compromising the image quality.
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spelling pubmed-85647272021-11-08 An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM) Ali, Rizwan El-Boubbou, Kheireddine Boudjelal, Mohamed MethodsX Method Article Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging. • An easy and rapid method to visualize biological specimens under SEM. • Cells are grown on carbon tapes and gold coated. • Air drying without compromising the image quality. Elsevier 2021-09-20 /pmc/articles/PMC8564727/ /pubmed/34754792 http://dx.doi.org/10.1016/j.mex.2021.101521 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Method Article
Ali, Rizwan
El-Boubbou, Kheireddine
Boudjelal, Mohamed
An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title_full An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title_fullStr An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title_full_unstemmed An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title_short An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)
title_sort easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (sem)
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564727/
https://www.ncbi.nlm.nih.gov/pubmed/34754792
http://dx.doi.org/10.1016/j.mex.2021.101521
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