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A standard knockout procedure alters expression of adjacent loci at the translational level

The Saccharomyces cerevisiae gene deletion collection is widely used for functional gene annotation and genetic interaction analyses. However, the standard G418-resistance cassette used to produce knockout mutants delivers strong regulatory elements into the target genetic loci. To date, its side ef...

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Detalles Bibliográficos
Autores principales:	Egorov, Artyom A, Alexandrov, Alexander I, Urakov, Valery N, Makeeva, Desislava S, Edakin, Roman O, Kushchenko, Artem S, Gladyshev, Vadim N, Kulakovskiy, Ivan V, Dmitriev, Sergey E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Genomics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565318/ https://www.ncbi.nlm.nih.gov/pubmed/34606617 http://dx.doi.org/10.1093/nar/gkab872

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