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Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas

CRISPR-Cas is a powerful tool for genome editing in bacteria. However, its efficacy is dependent on host factors (such as DNA repair pathways) and/or exogenous expression of recombinases. In this study, we mitigated these constraints by developing a simple and widely applicable genome engineering to...

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Detalles Bibliográficos
Autores principales:	Patinios, Constantinos, Creutzburg, Sjoerd C A, Arifah, Adini Q, Adiego-Pérez, Belén, Gyimah, Evans A, Ingham, Colin J, Kengen, Servé W M, van der Oost, John, Staals, Raymond H J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565351/ https://www.ncbi.nlm.nih.gov/pubmed/34614191 http://dx.doi.org/10.1093/nar/gkab893

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