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Integrator enforces the fidelity of transcriptional termination at protein-coding genes

Integrator regulates the 3′-end processing and termination of multiple classes of noncoding RNAs. Depletion of INTS11, the catalytic subunit of Integrator, or ectopic expression of its catalytic dead enzyme impairs the 3′-end processing and termination of a set of protein-coding transcripts termed I...

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Detalles Bibliográficos
Autores principales: Dasilva, Lucas Ferreira, Blumenthal, Ezra, Beckedorff, Felipe, Cingaram, Pradeep Reddy, Gomes Dos Santos, Helena, Edupuganti, Raghu Ram, Zhang, Anda, Dokaneheifard, Sadat, Aoi, Yuki, Yue, Jingyin, Kirstein, Nina, Tayari, Mina Masoumeh, Shilatifard, Ali, Shiekhattar, Ramin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565846/
https://www.ncbi.nlm.nih.gov/pubmed/34730992
http://dx.doi.org/10.1126/sciadv.abe3393
Descripción
Sumario:Integrator regulates the 3′-end processing and termination of multiple classes of noncoding RNAs. Depletion of INTS11, the catalytic subunit of Integrator, or ectopic expression of its catalytic dead enzyme impairs the 3′-end processing and termination of a set of protein-coding transcripts termed Integrator-regulated termination (IRT) genes. This defect is manifested by increased RNA polymerase II (RNAPII) readthrough and occupancy of serine-2 phosphorylated RNAPII, de novo trimethylation of lysine-36 on histone H3, and a compensatory elevation of the cleavage and polyadenylation (CPA) complex beyond the canonical polyadenylation sites. 3′ RNA sequencing reveals that proximal polyadenylation site usage relies on the endonuclease activity of INTS11. The DNA sequence encompassing the transcription end sites of IRT genes features downstream polyadenylation motifs and an enrichment of GC content that permits the formation of secondary structures within the 3′UTR. Together, this study identifies a subset of protein-coding transcripts whose 3′ end processing requires the Integrator complex.