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RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566248/ https://www.ncbi.nlm.nih.gov/pubmed/34618059 http://dx.doi.org/10.1093/plphys/kiab366 |
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author | Lv, Xinmin Sun, Yaqiang Hao, Pengbo Zhang, Cankui Tian, Ji Fu, Mengmeng Xu, Zhen Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai |
author_facet | Lv, Xinmin Sun, Yaqiang Hao, Pengbo Zhang, Cankui Tian, Ji Fu, Mengmeng Xu, Zhen Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai |
author_sort | Lv, Xinmin |
collection | PubMed |
description | Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that apple (Malus domestica) oligopeptide transporter3 (MdOPT3) mRNA can be transported over a long distance, from the leaf to the root, to regulate iron uptake; however, the mRNA of Arabidopsis (Arabidopsis thaliana) oligopeptide transporter 3 (AtOPT3), the MdOPT3 homolog from A. thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 became mobile and moved from the shoot to the root in two woody species, Malus and Populus, while the mobile MdOPT3 became immobile in two herbaceous species, A. thaliana and tomato (Solanum lycopersicum). Furthermore, we demonstrated that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by divergence in RNA-binding proteins between herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process. |
format | Online Article Text |
id | pubmed-8566248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-85662482021-11-04 RBP differentiation contributes to selective transmissibility of OPT3 mRNAs Lv, Xinmin Sun, Yaqiang Hao, Pengbo Zhang, Cankui Tian, Ji Fu, Mengmeng Xu, Zhen Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai Plant Physiol Regular Issue Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that apple (Malus domestica) oligopeptide transporter3 (MdOPT3) mRNA can be transported over a long distance, from the leaf to the root, to regulate iron uptake; however, the mRNA of Arabidopsis (Arabidopsis thaliana) oligopeptide transporter 3 (AtOPT3), the MdOPT3 homolog from A. thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 became mobile and moved from the shoot to the root in two woody species, Malus and Populus, while the mobile MdOPT3 became immobile in two herbaceous species, A. thaliana and tomato (Solanum lycopersicum). Furthermore, we demonstrated that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by divergence in RNA-binding proteins between herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process. Oxford University Press 2021-08-02 /pmc/articles/PMC8566248/ /pubmed/34618059 http://dx.doi.org/10.1093/plphys/kiab366 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Regular Issue Lv, Xinmin Sun, Yaqiang Hao, Pengbo Zhang, Cankui Tian, Ji Fu, Mengmeng Xu, Zhen Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title | RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title_full | RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title_fullStr | RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title_full_unstemmed | RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title_short | RBP differentiation contributes to selective transmissibility of OPT3 mRNAs |
title_sort | rbp differentiation contributes to selective transmissibility of opt3 mrnas |
topic | Regular Issue |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566248/ https://www.ncbi.nlm.nih.gov/pubmed/34618059 http://dx.doi.org/10.1093/plphys/kiab366 |
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