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RBP differentiation contributes to selective transmissibility of OPT3 mRNAs

Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes a...

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Autores principales: Lv, Xinmin, Sun, Yaqiang, Hao, Pengbo, Zhang, Cankui, Tian, Ji, Fu, Mengmeng, Xu, Zhen, Wang, Yi, Zhang, Xinzhong, Xu, Xuefeng, Wu, Ting, Han, Zhenhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566248/
https://www.ncbi.nlm.nih.gov/pubmed/34618059
http://dx.doi.org/10.1093/plphys/kiab366
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author Lv, Xinmin
Sun, Yaqiang
Hao, Pengbo
Zhang, Cankui
Tian, Ji
Fu, Mengmeng
Xu, Zhen
Wang, Yi
Zhang, Xinzhong
Xu, Xuefeng
Wu, Ting
Han, Zhenhai
author_facet Lv, Xinmin
Sun, Yaqiang
Hao, Pengbo
Zhang, Cankui
Tian, Ji
Fu, Mengmeng
Xu, Zhen
Wang, Yi
Zhang, Xinzhong
Xu, Xuefeng
Wu, Ting
Han, Zhenhai
author_sort Lv, Xinmin
collection PubMed
description Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that apple (Malus domestica) oligopeptide transporter3 (MdOPT3) mRNA can be transported over a long distance, from the leaf to the root, to regulate iron uptake; however, the mRNA of Arabidopsis (Arabidopsis thaliana) oligopeptide transporter 3 (AtOPT3), the MdOPT3 homolog from A. thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 became mobile and moved from the shoot to the root in two woody species, Malus and Populus, while the mobile MdOPT3 became immobile in two herbaceous species, A. thaliana and tomato (Solanum lycopersicum). Furthermore, we demonstrated that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by divergence in RNA-binding proteins between herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process.
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spelling pubmed-85662482021-11-04 RBP differentiation contributes to selective transmissibility of OPT3 mRNAs Lv, Xinmin Sun, Yaqiang Hao, Pengbo Zhang, Cankui Tian, Ji Fu, Mengmeng Xu, Zhen Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai Plant Physiol Regular Issue Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that apple (Malus domestica) oligopeptide transporter3 (MdOPT3) mRNA can be transported over a long distance, from the leaf to the root, to regulate iron uptake; however, the mRNA of Arabidopsis (Arabidopsis thaliana) oligopeptide transporter 3 (AtOPT3), the MdOPT3 homolog from A. thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 became mobile and moved from the shoot to the root in two woody species, Malus and Populus, while the mobile MdOPT3 became immobile in two herbaceous species, A. thaliana and tomato (Solanum lycopersicum). Furthermore, we demonstrated that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by divergence in RNA-binding proteins between herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process. Oxford University Press 2021-08-02 /pmc/articles/PMC8566248/ /pubmed/34618059 http://dx.doi.org/10.1093/plphys/kiab366 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Regular Issue
Lv, Xinmin
Sun, Yaqiang
Hao, Pengbo
Zhang, Cankui
Tian, Ji
Fu, Mengmeng
Xu, Zhen
Wang, Yi
Zhang, Xinzhong
Xu, Xuefeng
Wu, Ting
Han, Zhenhai
RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title_full RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title_fullStr RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title_full_unstemmed RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title_short RBP differentiation contributes to selective transmissibility of OPT3 mRNAs
title_sort rbp differentiation contributes to selective transmissibility of opt3 mrnas
topic Regular Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566248/
https://www.ncbi.nlm.nih.gov/pubmed/34618059
http://dx.doi.org/10.1093/plphys/kiab366
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