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A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)

Along with sexual maturity, the liver undergoes numerous metabolic processes to adapt the physiological changes associated with egg-laying in hens. However, mechanisms regulating the processes were unclear. In this study, comparative hepatic proteome and acetyl-proteome between pre- and peak-laying...

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Autores principales: Wang, Zhang, Wang, Dandan, Jiang, Keren, Guo, Yulong, Li, Zhuanjian, Jiang, Ruirui, Han, Ruili, Li, Guoxi, Tian, Yadong, Li, Hong, Kang, Xiangtao, Liu, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566343/
https://www.ncbi.nlm.nih.gov/pubmed/34746273
http://dx.doi.org/10.3389/fvets.2021.700669
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author Wang, Zhang
Wang, Dandan
Jiang, Keren
Guo, Yulong
Li, Zhuanjian
Jiang, Ruirui
Han, Ruili
Li, Guoxi
Tian, Yadong
Li, Hong
Kang, Xiangtao
Liu, Xiaojun
author_facet Wang, Zhang
Wang, Dandan
Jiang, Keren
Guo, Yulong
Li, Zhuanjian
Jiang, Ruirui
Han, Ruili
Li, Guoxi
Tian, Yadong
Li, Hong
Kang, Xiangtao
Liu, Xiaojun
author_sort Wang, Zhang
collection PubMed
description Along with sexual maturity, the liver undergoes numerous metabolic processes to adapt the physiological changes associated with egg-laying in hens. However, mechanisms regulating the processes were unclear. In this study, comparative hepatic proteome and acetyl-proteome between pre- and peak-laying hens were performed. The results showed that the upregulated proteins were mainly related to lipid and protein biosynthesis, while the downregulated proteins were mainly involved in pyruvate metabolism and were capable of inhibiting gluconeogenesis and lactate synthesis in peak-laying hens compared with that in pre-laying hens. With unchanged expression level, the significant acetylated proteins were largely functioned on activation of polyunsaturated fatty acid oxidation in peroxisome, while the significant deacetylated proteins were principally used to elevate medium and short fatty acid oxidation in mitochondria and oxidative phosphorylation. Most of the proteins which involved in gluconeogenesis, lipid transport, and detoxification were influenced by both protein expression and acetylation. Taken overall, a novel mechanism wherein an alternate source of acetyl coenzyme A was produced by activation of FA oxidation and pyruvate metabolism to meet the increased energy demand and lipid synthesis in liver of laying hens was uncovered. This study provides new insights into molecular mechanism of adaptation to physiological changes in liver of laying hens.
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spelling pubmed-85663432021-11-05 A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus) Wang, Zhang Wang, Dandan Jiang, Keren Guo, Yulong Li, Zhuanjian Jiang, Ruirui Han, Ruili Li, Guoxi Tian, Yadong Li, Hong Kang, Xiangtao Liu, Xiaojun Front Vet Sci Veterinary Science Along with sexual maturity, the liver undergoes numerous metabolic processes to adapt the physiological changes associated with egg-laying in hens. However, mechanisms regulating the processes were unclear. In this study, comparative hepatic proteome and acetyl-proteome between pre- and peak-laying hens were performed. The results showed that the upregulated proteins were mainly related to lipid and protein biosynthesis, while the downregulated proteins were mainly involved in pyruvate metabolism and were capable of inhibiting gluconeogenesis and lactate synthesis in peak-laying hens compared with that in pre-laying hens. With unchanged expression level, the significant acetylated proteins were largely functioned on activation of polyunsaturated fatty acid oxidation in peroxisome, while the significant deacetylated proteins were principally used to elevate medium and short fatty acid oxidation in mitochondria and oxidative phosphorylation. Most of the proteins which involved in gluconeogenesis, lipid transport, and detoxification were influenced by both protein expression and acetylation. Taken overall, a novel mechanism wherein an alternate source of acetyl coenzyme A was produced by activation of FA oxidation and pyruvate metabolism to meet the increased energy demand and lipid synthesis in liver of laying hens was uncovered. This study provides new insights into molecular mechanism of adaptation to physiological changes in liver of laying hens. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8566343/ /pubmed/34746273 http://dx.doi.org/10.3389/fvets.2021.700669 Text en Copyright © 2021 Wang, Wang, Jiang, Guo, Li, Jiang, Han, Li, Tian, Li, Kang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wang, Zhang
Wang, Dandan
Jiang, Keren
Guo, Yulong
Li, Zhuanjian
Jiang, Ruirui
Han, Ruili
Li, Guoxi
Tian, Yadong
Li, Hong
Kang, Xiangtao
Liu, Xiaojun
A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title_full A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title_fullStr A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title_full_unstemmed A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title_short A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Gallus gallus)
title_sort comprehensive proteome and acetyl-proteome atlas reveals molecular mechanisms adapting to the physiological changes from pre-laying to peak-laying stage in liver of hens (gallus gallus)
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566343/
https://www.ncbi.nlm.nih.gov/pubmed/34746273
http://dx.doi.org/10.3389/fvets.2021.700669
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