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Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit
At chemical synapses, neurotransmitters are packaged into synaptic vesicles that release their contents in response to depolarization. Despite its central role in synaptic function, regulation of the machinery that loads vesicles with neurotransmitters remains poorly understood. We find that synapti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566550/ https://www.ncbi.nlm.nih.gov/pubmed/34732711 http://dx.doi.org/10.1038/s41467-021-26575-3 |
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author | Choi, Jung-Hwan Horowitz, Lauren Bayer Ringstad, Niels |
author_facet | Choi, Jung-Hwan Horowitz, Lauren Bayer Ringstad, Niels |
author_sort | Choi, Jung-Hwan |
collection | PubMed |
description | At chemical synapses, neurotransmitters are packaged into synaptic vesicles that release their contents in response to depolarization. Despite its central role in synaptic function, regulation of the machinery that loads vesicles with neurotransmitters remains poorly understood. We find that synaptic glutamate signaling in a C. elegans chemosensory circuit is regulated by antagonistic interactions between the canonical vesicular glutamate transporter EAT-4/VGLUT and another vesicular transporter, VST-1. Loss of VST-1 strongly potentiates glutamate release from chemosensory BAG neurons and disrupts chemotaxis behavior. Analysis of the circuitry downstream of BAG neurons shows that excess glutamate release disrupts behavior by inappropriately recruiting RIA interneurons to the BAG-associated chemotaxis circuit. Our data indicate that in vivo the strength of glutamatergic synapses is controlled by regulation of neurotransmitter packaging into synaptic vesicles via functional coupling of VGLUT and VST-1. |
format | Online Article Text |
id | pubmed-8566550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85665502021-11-19 Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit Choi, Jung-Hwan Horowitz, Lauren Bayer Ringstad, Niels Nat Commun Article At chemical synapses, neurotransmitters are packaged into synaptic vesicles that release their contents in response to depolarization. Despite its central role in synaptic function, regulation of the machinery that loads vesicles with neurotransmitters remains poorly understood. We find that synaptic glutamate signaling in a C. elegans chemosensory circuit is regulated by antagonistic interactions between the canonical vesicular glutamate transporter EAT-4/VGLUT and another vesicular transporter, VST-1. Loss of VST-1 strongly potentiates glutamate release from chemosensory BAG neurons and disrupts chemotaxis behavior. Analysis of the circuitry downstream of BAG neurons shows that excess glutamate release disrupts behavior by inappropriately recruiting RIA interneurons to the BAG-associated chemotaxis circuit. Our data indicate that in vivo the strength of glutamatergic synapses is controlled by regulation of neurotransmitter packaging into synaptic vesicles via functional coupling of VGLUT and VST-1. Nature Publishing Group UK 2021-11-03 /pmc/articles/PMC8566550/ /pubmed/34732711 http://dx.doi.org/10.1038/s41467-021-26575-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Choi, Jung-Hwan Horowitz, Lauren Bayer Ringstad, Niels Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title | Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title_full | Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title_fullStr | Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title_full_unstemmed | Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title_short | Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit |
title_sort | opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a c. elegans sensory circuit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566550/ https://www.ncbi.nlm.nih.gov/pubmed/34732711 http://dx.doi.org/10.1038/s41467-021-26575-3 |
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