Cargando…
High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut
The cultivated peanut (Arachis hypogaea L.), which is rich in edible oil and protein, is widely planted around the world as an oil and cash crop. However, aflatoxin contamination seriously affects the quality safety of peanuts, hindering the development of the peanut industry and threatening the hea...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566722/ https://www.ncbi.nlm.nih.gov/pubmed/34745176 http://dx.doi.org/10.3389/fpls.2021.745408 |
_version_ | 1784594074865500160 |
---|---|
author | Jiang, Yifei Luo, Huaiyong Yu, Bolun Ding, Yingbin Kang, Yanping Huang, Li Zhou, Xiaojing Liu, Nian Chen, Weigang Guo, Jianbin Huai, Dongxin Lei, Yong Jiang, Huifang Yan, Liying Liao, Boshou |
author_facet | Jiang, Yifei Luo, Huaiyong Yu, Bolun Ding, Yingbin Kang, Yanping Huang, Li Zhou, Xiaojing Liu, Nian Chen, Weigang Guo, Jianbin Huai, Dongxin Lei, Yong Jiang, Huifang Yan, Liying Liao, Boshou |
author_sort | Jiang, Yifei |
collection | PubMed |
description | The cultivated peanut (Arachis hypogaea L.), which is rich in edible oil and protein, is widely planted around the world as an oil and cash crop. However, aflatoxin contamination seriously affects the quality safety of peanuts, hindering the development of the peanut industry and threatening the health of consumers. Breeding peanut varieties with resistance to Aspergillus flavus infection is important for the control of aflatoxin contamination, and understanding the genetic basis of resistance is vital to its genetic enhancement. In this study, we reported the quantitative trait locus (QTL) mapping of resistance to A. flavus infection of a well-known resistant variety, J11. A mapping population consisting of 200 recombinant inbred lines (RILs) was constructed by crossing a susceptible variety, Zhonghua 16, with J11. Through whole-genome resequencing, a genetic linkage map was constructed with 2,802 recombination bins and an average inter-bin distance of 0.58 cM. Combined with phenotypic data of an infection index in 4 consecutive years, six novel resistant QTLs with 5.03–10.87% phenotypic variances explained (PVE) were identified on chromosomes A05, A08, B01, B03, and B10. The favorable alleles of five QTLs were from J11, while that of one QTL was from Zhonghua 16. The combination of these favorable alleles significantly improved resistance to A. flavus infection. These results could contribute greatly to the understanding of the genetic basis of A. flavus resistance and could be meaningful in the improvement of further resistance in peanuts. |
format | Online Article Text |
id | pubmed-8566722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85667222021-11-05 High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut Jiang, Yifei Luo, Huaiyong Yu, Bolun Ding, Yingbin Kang, Yanping Huang, Li Zhou, Xiaojing Liu, Nian Chen, Weigang Guo, Jianbin Huai, Dongxin Lei, Yong Jiang, Huifang Yan, Liying Liao, Boshou Front Plant Sci Plant Science The cultivated peanut (Arachis hypogaea L.), which is rich in edible oil and protein, is widely planted around the world as an oil and cash crop. However, aflatoxin contamination seriously affects the quality safety of peanuts, hindering the development of the peanut industry and threatening the health of consumers. Breeding peanut varieties with resistance to Aspergillus flavus infection is important for the control of aflatoxin contamination, and understanding the genetic basis of resistance is vital to its genetic enhancement. In this study, we reported the quantitative trait locus (QTL) mapping of resistance to A. flavus infection of a well-known resistant variety, J11. A mapping population consisting of 200 recombinant inbred lines (RILs) was constructed by crossing a susceptible variety, Zhonghua 16, with J11. Through whole-genome resequencing, a genetic linkage map was constructed with 2,802 recombination bins and an average inter-bin distance of 0.58 cM. Combined with phenotypic data of an infection index in 4 consecutive years, six novel resistant QTLs with 5.03–10.87% phenotypic variances explained (PVE) were identified on chromosomes A05, A08, B01, B03, and B10. The favorable alleles of five QTLs were from J11, while that of one QTL was from Zhonghua 16. The combination of these favorable alleles significantly improved resistance to A. flavus infection. These results could contribute greatly to the understanding of the genetic basis of A. flavus resistance and could be meaningful in the improvement of further resistance in peanuts. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8566722/ /pubmed/34745176 http://dx.doi.org/10.3389/fpls.2021.745408 Text en Copyright © 2021 Jiang, Luo, Yu, Ding, Kang, Huang, Zhou, Liu, Chen, Guo, Huai, Lei, Jiang, Yan and Liao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Jiang, Yifei Luo, Huaiyong Yu, Bolun Ding, Yingbin Kang, Yanping Huang, Li Zhou, Xiaojing Liu, Nian Chen, Weigang Guo, Jianbin Huai, Dongxin Lei, Yong Jiang, Huifang Yan, Liying Liao, Boshou High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title | High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title_full | High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title_fullStr | High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title_full_unstemmed | High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title_short | High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping QTLs of Resistance to Aspergillus flavus Infection in Peanut |
title_sort | high-density genetic linkage map construction using whole-genome resequencing for mapping qtls of resistance to aspergillus flavus infection in peanut |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566722/ https://www.ncbi.nlm.nih.gov/pubmed/34745176 http://dx.doi.org/10.3389/fpls.2021.745408 |
work_keys_str_mv | AT jiangyifei highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT luohuaiyong highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT yubolun highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT dingyingbin highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT kangyanping highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT huangli highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT zhouxiaojing highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT liunian highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT chenweigang highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT guojianbin highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT huaidongxin highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT leiyong highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT jianghuifang highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT yanliying highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut AT liaoboshou highdensitygeneticlinkagemapconstructionusingwholegenomeresequencingformappingqtlsofresistancetoaspergillusflavusinfectioninpeanut |