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Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.

The taproot of radish (Raphanus sativus L.) is an important sink organ; it is morphologically diverse and contains large amounts of secondary metabolites. Sucrose metabolism is believed to be important in the development of sink organs. We measured the amounts of glucose, fructose, and sucrose in th...

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Autores principales: Kang, Ji-Nam, Kim, Jung Sun, Lee, Si Myung, Won, So Youn, Seo, Mi-Suk, Kwon, Soo-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566945/
https://www.ncbi.nlm.nih.gov/pubmed/34745157
http://dx.doi.org/10.3389/fpls.2021.716782
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author Kang, Ji-Nam
Kim, Jung Sun
Lee, Si Myung
Won, So Youn
Seo, Mi-Suk
Kwon, Soo-Jin
author_facet Kang, Ji-Nam
Kim, Jung Sun
Lee, Si Myung
Won, So Youn
Seo, Mi-Suk
Kwon, Soo-Jin
author_sort Kang, Ji-Nam
collection PubMed
description The taproot of radish (Raphanus sativus L.) is an important sink organ; it is morphologically diverse and contains large amounts of secondary metabolites. Sucrose metabolism is believed to be important in the development of sink organs. We measured the amounts of glucose, fructose, and sucrose in the roots of sixty three radish accessions and analyzed the association between the sugar content and the root phenotype. Fructose content correlated with the root color and length characteristics, glucose was the most abundant sugar in the roots, and the sucrose content was very low, compared to that of the hexoses in most of the accessions. Expression analysis of the genes involved in sucrose metabolism, transportation, starch synthesis, and cell wall synthesis was performed through RNA sequencing. The genes encoding sucrose synthases (SUSY) and the enzymes involved in the synthesis of cellulose were highly expressed, indicating that SUSY is involved in cell wall synthesis in radish roots. The positive correlation coefficient (R) between the sucrose content and the expression of cell wall invertase and sugar transporter proteins suggest that hexose accumulation could occur through the apoplastic pathway in radish roots. A positive R score was also obtained when comparing the expression of genes encoding SUSY and fructokinase (FK), suggesting that the fructose produced by SUSY is mostly phosphorylated by FK. In addition, we concluded that sucrose was the most metabolized sugar in radish roots.
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spelling pubmed-85669452021-11-05 Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L. Kang, Ji-Nam Kim, Jung Sun Lee, Si Myung Won, So Youn Seo, Mi-Suk Kwon, Soo-Jin Front Plant Sci Plant Science The taproot of radish (Raphanus sativus L.) is an important sink organ; it is morphologically diverse and contains large amounts of secondary metabolites. Sucrose metabolism is believed to be important in the development of sink organs. We measured the amounts of glucose, fructose, and sucrose in the roots of sixty three radish accessions and analyzed the association between the sugar content and the root phenotype. Fructose content correlated with the root color and length characteristics, glucose was the most abundant sugar in the roots, and the sucrose content was very low, compared to that of the hexoses in most of the accessions. Expression analysis of the genes involved in sucrose metabolism, transportation, starch synthesis, and cell wall synthesis was performed through RNA sequencing. The genes encoding sucrose synthases (SUSY) and the enzymes involved in the synthesis of cellulose were highly expressed, indicating that SUSY is involved in cell wall synthesis in radish roots. The positive correlation coefficient (R) between the sucrose content and the expression of cell wall invertase and sugar transporter proteins suggest that hexose accumulation could occur through the apoplastic pathway in radish roots. A positive R score was also obtained when comparing the expression of genes encoding SUSY and fructokinase (FK), suggesting that the fructose produced by SUSY is mostly phosphorylated by FK. In addition, we concluded that sucrose was the most metabolized sugar in radish roots. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8566945/ /pubmed/34745157 http://dx.doi.org/10.3389/fpls.2021.716782 Text en Copyright © 2021 Kang, Kim, Lee, Won, Seo and Kwon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kang, Ji-Nam
Kim, Jung Sun
Lee, Si Myung
Won, So Youn
Seo, Mi-Suk
Kwon, Soo-Jin
Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title_full Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title_fullStr Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title_full_unstemmed Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title_short Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.
title_sort analysis of phenotypic characteristics and sucrose metabolism in the roots of raphanus sativus l.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566945/
https://www.ncbi.nlm.nih.gov/pubmed/34745157
http://dx.doi.org/10.3389/fpls.2021.716782
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