Cargando…

Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke

Arterial hypertension is considered the most prevalent risk factor for stroke. Both pathophysiologic and clinical data previously acquired suggest a strong correlation between the hemodynamic nature of arterial hypertension and an increase in the risk of ischemic insult to tissues. However, the know...

Descripción completa

Detalles Bibliográficos
Autores principales: Mu, Qiancheng, Zhang, Yuxuan, Gu, Long, Gerner, Stefan T., Qiu, Xiancheng, Tao, Qianke, Pang, Jinwei, Dipritu, Ghosh, Zhang, Lifang, Yin, Shigang, Jiang, Yong, Peng, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566985/
https://www.ncbi.nlm.nih.gov/pubmed/34744970
http://dx.doi.org/10.3389/fneur.2021.728984
_version_ 1784594136331976704
author Mu, Qiancheng
Zhang, Yuxuan
Gu, Long
Gerner, Stefan T.
Qiu, Xiancheng
Tao, Qianke
Pang, Jinwei
Dipritu, Ghosh
Zhang, Lifang
Yin, Shigang
Jiang, Yong
Peng, Jianhua
author_facet Mu, Qiancheng
Zhang, Yuxuan
Gu, Long
Gerner, Stefan T.
Qiu, Xiancheng
Tao, Qianke
Pang, Jinwei
Dipritu, Ghosh
Zhang, Lifang
Yin, Shigang
Jiang, Yong
Peng, Jianhua
author_sort Mu, Qiancheng
collection PubMed
description Arterial hypertension is considered the most prevalent risk factor for stroke. Both pathophysiologic and clinical data previously acquired suggest a strong correlation between the hemodynamic nature of arterial hypertension and an increase in the risk of ischemic insult to tissues. However, the knowledge of specific molecular interactions between hypertension and ischemic stroke (IS) is limited. In this study, we performed systematic bioinformatics analysis of stroke-prone spontaneous hypertensive brain tissue samples of rats (GSE41452), middle cerebral artery occlusion of brain tissue samples of rats (GSE97537), and peripheral blood array data of IS patients (GSE22255). We identified that Fos, an immediate-early gene (IEG) that responds to alterations in arterial blood pressure, has a strong correlation with the occurrence and prognosis of IS. To further evaluate the potential function of Fos, the oxygen–glucose deprivation model and RNA sequencing of HT22 neuronal cells were performed. Consistent with the sequencing results, real-time quantitative PCR and Western blot indicate that Fos was elevated at 3 h and returned to normal levels at 6 h after oxygen–glucose deprivation. Knock-down of Fos by lentivirus significantly increased the oxidative stress level, neuronal apoptosis, and inhibited the mitochondrial function. In conclusion, Fos acts as an important link between hypertension and IS. Furthermore, Fos can be used as a potential biomarker for target therapy in the prevention of stroke among hypertensive patients and also potential treatment targeting apoptosis and oxidative stress after its onset.
format Online
Article
Text
id pubmed-8566985
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85669852021-11-05 Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke Mu, Qiancheng Zhang, Yuxuan Gu, Long Gerner, Stefan T. Qiu, Xiancheng Tao, Qianke Pang, Jinwei Dipritu, Ghosh Zhang, Lifang Yin, Shigang Jiang, Yong Peng, Jianhua Front Neurol Neurology Arterial hypertension is considered the most prevalent risk factor for stroke. Both pathophysiologic and clinical data previously acquired suggest a strong correlation between the hemodynamic nature of arterial hypertension and an increase in the risk of ischemic insult to tissues. However, the knowledge of specific molecular interactions between hypertension and ischemic stroke (IS) is limited. In this study, we performed systematic bioinformatics analysis of stroke-prone spontaneous hypertensive brain tissue samples of rats (GSE41452), middle cerebral artery occlusion of brain tissue samples of rats (GSE97537), and peripheral blood array data of IS patients (GSE22255). We identified that Fos, an immediate-early gene (IEG) that responds to alterations in arterial blood pressure, has a strong correlation with the occurrence and prognosis of IS. To further evaluate the potential function of Fos, the oxygen–glucose deprivation model and RNA sequencing of HT22 neuronal cells were performed. Consistent with the sequencing results, real-time quantitative PCR and Western blot indicate that Fos was elevated at 3 h and returned to normal levels at 6 h after oxygen–glucose deprivation. Knock-down of Fos by lentivirus significantly increased the oxidative stress level, neuronal apoptosis, and inhibited the mitochondrial function. In conclusion, Fos acts as an important link between hypertension and IS. Furthermore, Fos can be used as a potential biomarker for target therapy in the prevention of stroke among hypertensive patients and also potential treatment targeting apoptosis and oxidative stress after its onset. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8566985/ /pubmed/34744970 http://dx.doi.org/10.3389/fneur.2021.728984 Text en Copyright © 2021 Mu, Zhang, Gu, Gerner, Qiu, Tao, Pang, Dipritu, Zhang, Yin, Jiang and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Mu, Qiancheng
Zhang, Yuxuan
Gu, Long
Gerner, Stefan T.
Qiu, Xiancheng
Tao, Qianke
Pang, Jinwei
Dipritu, Ghosh
Zhang, Lifang
Yin, Shigang
Jiang, Yong
Peng, Jianhua
Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title_full Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title_fullStr Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title_full_unstemmed Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title_short Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
title_sort transcriptomic profiling reveals the antiapoptosis and antioxidant stress effects of fos in ischemic stroke
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566985/
https://www.ncbi.nlm.nih.gov/pubmed/34744970
http://dx.doi.org/10.3389/fneur.2021.728984
work_keys_str_mv AT muqiancheng transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT zhangyuxuan transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT gulong transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT gernerstefant transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT qiuxiancheng transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT taoqianke transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT pangjinwei transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT dipritughosh transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT zhanglifang transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT yinshigang transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT jiangyong transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke
AT pengjianhua transcriptomicprofilingrevealstheantiapoptosisandantioxidantstresseffectsoffosinischemicstroke