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The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer

Tumorigenesis refers to the process of clonal dysplasia that occurs due to the collapse of normal growth regulation in cells caused by the action of various carcinogenic factors. These “successful” tumor cells pass on the genetic templates to their generations in evolutionary terms, but they also co...

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Autores principales: Li, Jing, Yu, Nan, Li, Xin, Cui, Mengna, Guo, Qie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566994/
https://www.ncbi.nlm.nih.gov/pubmed/34745998
http://dx.doi.org/10.3389/fonc.2021.759894
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author Li, Jing
Yu, Nan
Li, Xin
Cui, Mengna
Guo, Qie
author_facet Li, Jing
Yu, Nan
Li, Xin
Cui, Mengna
Guo, Qie
author_sort Li, Jing
collection PubMed
description Tumorigenesis refers to the process of clonal dysplasia that occurs due to the collapse of normal growth regulation in cells caused by the action of various carcinogenic factors. These “successful” tumor cells pass on the genetic templates to their generations in evolutionary terms, but they also constantly adapt to ever-changing host environments. A unique peculiarity known as intratumor heterogeneity (ITH) is extensively involved in tumor development, metastasis, chemoresistance, and immune escape. An understanding of ITH is urgently required to identify the diversity and complexity of the tumor microenvironment (TME), but achieving this understanding has been a challenge. Single-cell sequencing (SCS) is a powerful tool that can gauge the distribution of genomic sequences in a single cell and the genetic variability among tumor cells, which can improve the understanding of ITH. SCS provides fundamental ideas about existing diversity in specific TMEs, thus improving cancer diagnosis and prognosis prediction, as well as improving the monitoring of therapeutic response. Herein, we will discuss advances in SCS and review SCS application in tumors based on current evidence.
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spelling pubmed-85669942021-11-05 The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer Li, Jing Yu, Nan Li, Xin Cui, Mengna Guo, Qie Front Oncol Oncology Tumorigenesis refers to the process of clonal dysplasia that occurs due to the collapse of normal growth regulation in cells caused by the action of various carcinogenic factors. These “successful” tumor cells pass on the genetic templates to their generations in evolutionary terms, but they also constantly adapt to ever-changing host environments. A unique peculiarity known as intratumor heterogeneity (ITH) is extensively involved in tumor development, metastasis, chemoresistance, and immune escape. An understanding of ITH is urgently required to identify the diversity and complexity of the tumor microenvironment (TME), but achieving this understanding has been a challenge. Single-cell sequencing (SCS) is a powerful tool that can gauge the distribution of genomic sequences in a single cell and the genetic variability among tumor cells, which can improve the understanding of ITH. SCS provides fundamental ideas about existing diversity in specific TMEs, thus improving cancer diagnosis and prognosis prediction, as well as improving the monitoring of therapeutic response. Herein, we will discuss advances in SCS and review SCS application in tumors based on current evidence. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8566994/ /pubmed/34745998 http://dx.doi.org/10.3389/fonc.2021.759894 Text en Copyright © 2021 Li, Yu, Li, Cui and Guo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Jing
Yu, Nan
Li, Xin
Cui, Mengna
Guo, Qie
The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title_full The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title_fullStr The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title_full_unstemmed The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title_short The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer
title_sort single-cell sequencing: a dazzling light shining on the dark corner of cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566994/
https://www.ncbi.nlm.nih.gov/pubmed/34745998
http://dx.doi.org/10.3389/fonc.2021.759894
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