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Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei

Solanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes...

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Autores principales: Bautista, Daniel, Guayazan-Palacios, Natalia, Buitrago, Maria Camila, Cardenas, Martha, Botero, David, Duitama, Jorge, Bernal, Adriana J., Restrepo, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567061/
https://www.ncbi.nlm.nih.gov/pubmed/34745164
http://dx.doi.org/10.3389/fpls.2021.730251
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author Bautista, Daniel
Guayazan-Palacios, Natalia
Buitrago, Maria Camila
Cardenas, Martha
Botero, David
Duitama, Jorge
Bernal, Adriana J.
Restrepo, Silvia
author_facet Bautista, Daniel
Guayazan-Palacios, Natalia
Buitrago, Maria Camila
Cardenas, Martha
Botero, David
Duitama, Jorge
Bernal, Adriana J.
Restrepo, Silvia
author_sort Bautista, Daniel
collection PubMed
description Solanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes late blight in S. betaceum. There is no general study of the response of S. betaceum, particularly, in the changes in expression of pathogenesis-related genes. In this manuscript we present a comprehensive RNA-seq time-series study of the plant response to the infection of P. betacei. Following six time points of infection, the differentially expressed genes (DEGs) involved in the defense by the plant were contextualized in a sequential manner. We documented 5,628 DEGs across all time-points. From 6 to 24 h post-inoculation, we highlighted DEGs involved in the recognition of the pathogen by the likely activation of pattern-triggered immunity (PTI) genes. We also describe the possible effect of the pathogen effectors in the host during the effector-triggered response. Finally, we reveal genes related to the susceptible outcome of the interaction caused by the onset of necrotrophy and the sharp transcriptional changes as a response to the pathogen. This is the first report of the transcriptome of the tree tomato in response to the newly described pathogen P. betacei.
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spelling pubmed-85670612021-11-05 Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei Bautista, Daniel Guayazan-Palacios, Natalia Buitrago, Maria Camila Cardenas, Martha Botero, David Duitama, Jorge Bernal, Adriana J. Restrepo, Silvia Front Plant Sci Plant Science Solanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes late blight in S. betaceum. There is no general study of the response of S. betaceum, particularly, in the changes in expression of pathogenesis-related genes. In this manuscript we present a comprehensive RNA-seq time-series study of the plant response to the infection of P. betacei. Following six time points of infection, the differentially expressed genes (DEGs) involved in the defense by the plant were contextualized in a sequential manner. We documented 5,628 DEGs across all time-points. From 6 to 24 h post-inoculation, we highlighted DEGs involved in the recognition of the pathogen by the likely activation of pattern-triggered immunity (PTI) genes. We also describe the possible effect of the pathogen effectors in the host during the effector-triggered response. Finally, we reveal genes related to the susceptible outcome of the interaction caused by the onset of necrotrophy and the sharp transcriptional changes as a response to the pathogen. This is the first report of the transcriptome of the tree tomato in response to the newly described pathogen P. betacei. Frontiers Media S.A. 2021-10-21 /pmc/articles/PMC8567061/ /pubmed/34745164 http://dx.doi.org/10.3389/fpls.2021.730251 Text en Copyright © 2021 Bautista, Guayazan-Palacios, Buitrago, Cardenas, Botero, Duitama, Bernal and Restrepo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bautista, Daniel
Guayazan-Palacios, Natalia
Buitrago, Maria Camila
Cardenas, Martha
Botero, David
Duitama, Jorge
Bernal, Adriana J.
Restrepo, Silvia
Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title_full Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title_fullStr Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title_full_unstemmed Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title_short Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
title_sort comprehensive time-series analysis of the gene expression profile in a susceptible cultivar of tree tomato (solanum betaceum) during the infection of phytophthora betacei
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567061/
https://www.ncbi.nlm.nih.gov/pubmed/34745164
http://dx.doi.org/10.3389/fpls.2021.730251
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