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Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer
[Image: see text] In this study, a medical-grade poly(l-lactide-co-ε-caprolactone) (PLC) copolymer with a monomer ratio of l-lactide (L) to ε-caprolactone (C) of 70:30 mol % for use as an absorbable surgical suture was synthesized via ring-opening polymerization (ROP) using a novel soluble liquid ti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567407/ https://www.ncbi.nlm.nih.gov/pubmed/34746572 http://dx.doi.org/10.1021/acsomega.1c03569 |
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author | Sriyai, Montira Tasati, Jagkrit Molloy, Robert Meepowpan, Puttinan Somsunan, Runglawan Worajittiphon, Patnarin Daranarong, Donraporn Meerak, Jomkwan Punyodom, Winita |
author_facet | Sriyai, Montira Tasati, Jagkrit Molloy, Robert Meepowpan, Puttinan Somsunan, Runglawan Worajittiphon, Patnarin Daranarong, Donraporn Meerak, Jomkwan Punyodom, Winita |
author_sort | Sriyai, Montira |
collection | PubMed |
description | [Image: see text] In this study, a medical-grade poly(l-lactide-co-ε-caprolactone) (PLC) copolymer with a monomer ratio of l-lactide (L) to ε-caprolactone (C) of 70:30 mol % for use as an absorbable surgical suture was synthesized via ring-opening polymerization (ROP) using a novel soluble liquid tin(II) n-butoxide (Sn(OnC(4)H(9))(2)) as an initiator. In fiber fabrication, the process included copolymer melt extrusion with a minimal draw followed by sequential controlled hot-drawing and fixed-annealing steps to obtain oriented semicrystalline fibers with improved mechanical strength. For healing enhancement, the fiber was dip-coated with “levofloxacin” by adding the drug into a solution mixture of acetone, poly(ε-caprolactone) (PCL), and calcium stearate (CaSt) in the ratio of acetone/PCL/CaSt = 100:1% w/v:0.1% w/v. The tensile strength of the coated fiber was found to be increased to ∼400 MPa, which is comparable with that of commercial polydioxanone (PDS II) of a similar size. Finally, the efficiency of the drug-coated fiber regarding its controlled drug release and antimicrobial activity was investigated, and the results showed that the coated fiber was able to release the drug continuously for as long as 30 days. For fiber antimicrobial activity, it was found that a concentration of 1 mg/mL was sufficient to inhibit the growth of Staphylococcus aureus (MRSA), Escherichia coli O157:H7, and Pseudomonas aeruginosa, giving a clear inhibition zone range of 20–24 mm for 90 days. Cytotoxicity testing of the drug-coated fibers showed a %viability of more than 70%, indicating that they were nontoxic. |
format | Online Article Text |
id | pubmed-8567407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-85674072021-11-05 Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer Sriyai, Montira Tasati, Jagkrit Molloy, Robert Meepowpan, Puttinan Somsunan, Runglawan Worajittiphon, Patnarin Daranarong, Donraporn Meerak, Jomkwan Punyodom, Winita ACS Omega [Image: see text] In this study, a medical-grade poly(l-lactide-co-ε-caprolactone) (PLC) copolymer with a monomer ratio of l-lactide (L) to ε-caprolactone (C) of 70:30 mol % for use as an absorbable surgical suture was synthesized via ring-opening polymerization (ROP) using a novel soluble liquid tin(II) n-butoxide (Sn(OnC(4)H(9))(2)) as an initiator. In fiber fabrication, the process included copolymer melt extrusion with a minimal draw followed by sequential controlled hot-drawing and fixed-annealing steps to obtain oriented semicrystalline fibers with improved mechanical strength. For healing enhancement, the fiber was dip-coated with “levofloxacin” by adding the drug into a solution mixture of acetone, poly(ε-caprolactone) (PCL), and calcium stearate (CaSt) in the ratio of acetone/PCL/CaSt = 100:1% w/v:0.1% w/v. The tensile strength of the coated fiber was found to be increased to ∼400 MPa, which is comparable with that of commercial polydioxanone (PDS II) of a similar size. Finally, the efficiency of the drug-coated fiber regarding its controlled drug release and antimicrobial activity was investigated, and the results showed that the coated fiber was able to release the drug continuously for as long as 30 days. For fiber antimicrobial activity, it was found that a concentration of 1 mg/mL was sufficient to inhibit the growth of Staphylococcus aureus (MRSA), Escherichia coli O157:H7, and Pseudomonas aeruginosa, giving a clear inhibition zone range of 20–24 mm for 90 days. Cytotoxicity testing of the drug-coated fibers showed a %viability of more than 70%, indicating that they were nontoxic. American Chemical Society 2021-10-25 /pmc/articles/PMC8567407/ /pubmed/34746572 http://dx.doi.org/10.1021/acsomega.1c03569 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Sriyai, Montira Tasati, Jagkrit Molloy, Robert Meepowpan, Puttinan Somsunan, Runglawan Worajittiphon, Patnarin Daranarong, Donraporn Meerak, Jomkwan Punyodom, Winita Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title | Development of an Antimicrobial-Coated Absorbable
Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title_full | Development of an Antimicrobial-Coated Absorbable
Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title_fullStr | Development of an Antimicrobial-Coated Absorbable
Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title_full_unstemmed | Development of an Antimicrobial-Coated Absorbable
Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title_short | Development of an Antimicrobial-Coated Absorbable
Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer |
title_sort | development of an antimicrobial-coated absorbable
monofilament suture from a medical-grade poly(l-lactide-co-ε-caprolactone) copolymer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567407/ https://www.ncbi.nlm.nih.gov/pubmed/34746572 http://dx.doi.org/10.1021/acsomega.1c03569 |
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