Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings

Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficie...

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Detalles Bibliográficos
Autores principales: Liu, Yanlin, Xiong, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567432/
https://www.ncbi.nlm.nih.gov/pubmed/34761234
http://dx.doi.org/10.1016/j.xpro.2021.100922
Descripción
Sumario:Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021).

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