Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings

Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficie...

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Detalles Bibliográficos
Autores principales: Liu, Yanlin, Xiong, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567432/
https://www.ncbi.nlm.nih.gov/pubmed/34761234
http://dx.doi.org/10.1016/j.xpro.2021.100922
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author Liu, Yanlin
Xiong, Yan
author_facet Liu, Yanlin
Xiong, Yan
author_sort Liu, Yanlin
collection PubMed
description Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021).
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spelling pubmed-85674322021-11-09 Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings Liu, Yanlin Xiong, Yan STAR Protoc Protocol Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021). Elsevier 2021-10-29 /pmc/articles/PMC8567432/ /pubmed/34761234 http://dx.doi.org/10.1016/j.xpro.2021.100922 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Yanlin
Xiong, Yan
Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title_full Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title_fullStr Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title_full_unstemmed Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title_short Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
title_sort liquid culture system for efficient depletion of the endogenous nutrients in arabidopsis seedlings
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567432/
https://www.ncbi.nlm.nih.gov/pubmed/34761234
http://dx.doi.org/10.1016/j.xpro.2021.100922
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AT xiongyan liquidculturesystemforefficientdepletionoftheendogenousnutrientsinarabidopsisseedlings

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