In vitro evaluation of CAR-T cells in patient-derived glioblastoma models

Advances in chimeric antigen receptor (CAR) T cell therapies have led to the modality dominating translational cancer research; however, a standardized protocol for evaluating such therapies in vitro is needed. This protocol details the in vitro preclinical evaluation of CAR-T cell therapies for gli...

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Autores principales: Brakel, Benjamin A., Chokshi, Chirayu R., Salim, Sabra K., Venugopal, Chitra, Singh, Sheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567433/
https://www.ncbi.nlm.nih.gov/pubmed/34761232
http://dx.doi.org/10.1016/j.xpro.2021.100920
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author Brakel, Benjamin A.
Chokshi, Chirayu R.
Salim, Sabra K.
Venugopal, Chitra
Singh, Sheila
author_facet Brakel, Benjamin A.
Chokshi, Chirayu R.
Salim, Sabra K.
Venugopal, Chitra
Singh, Sheila
author_sort Brakel, Benjamin A.
collection PubMed
description Advances in chimeric antigen receptor (CAR) T cell therapies have led to the modality dominating translational cancer research; however, a standardized protocol for evaluating such therapies in vitro is needed. This protocol details the in vitro preclinical evaluation of CAR-T cell therapies for glioblastoma (GBM), including target cell cytotoxicity and T cell proliferation, activation, and cytokine release assays. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020).
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spelling pubmed-85674332021-11-09 In vitro evaluation of CAR-T cells in patient-derived glioblastoma models Brakel, Benjamin A. Chokshi, Chirayu R. Salim, Sabra K. Venugopal, Chitra Singh, Sheila STAR Protoc Protocol Advances in chimeric antigen receptor (CAR) T cell therapies have led to the modality dominating translational cancer research; however, a standardized protocol for evaluating such therapies in vitro is needed. This protocol details the in vitro preclinical evaluation of CAR-T cell therapies for glioblastoma (GBM), including target cell cytotoxicity and T cell proliferation, activation, and cytokine release assays. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020). Elsevier 2021-10-29 /pmc/articles/PMC8567433/ /pubmed/34761232 http://dx.doi.org/10.1016/j.xpro.2021.100920 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Brakel, Benjamin A.
Chokshi, Chirayu R.
Salim, Sabra K.
Venugopal, Chitra
Singh, Sheila
In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title_full In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title_fullStr In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title_full_unstemmed In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title_short In vitro evaluation of CAR-T cells in patient-derived glioblastoma models
title_sort in vitro evaluation of car-t cells in patient-derived glioblastoma models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567433/
https://www.ncbi.nlm.nih.gov/pubmed/34761232
http://dx.doi.org/10.1016/j.xpro.2021.100920
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