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A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples

Here, we describe a protocol for cell-based detection of autoantibodies from human plasma and serum samples using a standard flow cytometer. The protocol allows detection of autoantibodies against a wide array of extracellular antigens. Antigen coverage is limited to the cell types tested, and resea...

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Detalles Bibliográficos
Autores principales: Wong, Andrew Kam Ho, Kulpa, Deanna A., Silvestri, Guido, Maier, Cheryl L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567435/
https://www.ncbi.nlm.nih.gov/pubmed/34761236
http://dx.doi.org/10.1016/j.xpro.2021.100924