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Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
BACKGROUND: Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of protein r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567705/ https://www.ncbi.nlm.nih.gov/pubmed/34736417 http://dx.doi.org/10.1186/s12868-021-00668-7 |
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author | Seo, Min Hyung Lim, Sabina Yeo, Sujung |
author_facet | Seo, Min Hyung Lim, Sabina Yeo, Sujung |
author_sort | Seo, Min Hyung |
collection | PubMed |
description | BACKGROUND: Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of protein related to PD in TRDN knockdown status were studied in the SH-SY5Y cells. Cell viability and apoptosis were assessed to examine the apoptosis effect on dopaminergic cells by decreased TRDN, and the levels of the proteins related to apoptosis were also confirmed. RESULTS: This study confirmed decreased TRDN expression level (P < 0.005) at the SN in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model and identified the functional features of TRDN. Our results showed a relationship between TRDN expression and PD in that reduced TRDN level induced PD-like characteristics. Interestingly, there was TRDN expression in the regions where dopaminergic cells are in the SN, and the expression patterns of TRDN and tyrosine hydroxylase (TH) were similar. Decreased TRDN level also induced apoptotic characteristics and the Fluorescence-activated cell sorting analysis results showed that apoptosis increased (P < 0.05) as the TRDN small interfering RNA concentration increased. The cytotoxicity assay revealed that cell viability also decreased (P < 0.0005) in the same condition as that in the Fluorescence‐activated cell sorting analysis. CONCLUSIONS: Decreased TRDN level could be related with the apoptotic death of dopaminergic cells at the SN in PD, and TRDN administration could give a positive effect on PD by reducing apoptotic cell death. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12868-021-00668-7. |
format | Online Article Text |
id | pubmed-8567705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-85677052021-11-04 Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model Seo, Min Hyung Lim, Sabina Yeo, Sujung BMC Neurosci Research Article BACKGROUND: Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of protein related to PD in TRDN knockdown status were studied in the SH-SY5Y cells. Cell viability and apoptosis were assessed to examine the apoptosis effect on dopaminergic cells by decreased TRDN, and the levels of the proteins related to apoptosis were also confirmed. RESULTS: This study confirmed decreased TRDN expression level (P < 0.005) at the SN in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model and identified the functional features of TRDN. Our results showed a relationship between TRDN expression and PD in that reduced TRDN level induced PD-like characteristics. Interestingly, there was TRDN expression in the regions where dopaminergic cells are in the SN, and the expression patterns of TRDN and tyrosine hydroxylase (TH) were similar. Decreased TRDN level also induced apoptotic characteristics and the Fluorescence-activated cell sorting analysis results showed that apoptosis increased (P < 0.05) as the TRDN small interfering RNA concentration increased. The cytotoxicity assay revealed that cell viability also decreased (P < 0.0005) in the same condition as that in the Fluorescence‐activated cell sorting analysis. CONCLUSIONS: Decreased TRDN level could be related with the apoptotic death of dopaminergic cells at the SN in PD, and TRDN administration could give a positive effect on PD by reducing apoptotic cell death. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12868-021-00668-7. BioMed Central 2021-11-04 /pmc/articles/PMC8567705/ /pubmed/34736417 http://dx.doi.org/10.1186/s12868-021-00668-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Seo, Min Hyung Lim, Sabina Yeo, Sujung Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title | Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title_full | Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title_fullStr | Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title_full_unstemmed | Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title_short | Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model |
title_sort | association of decreased triadin expression level with apoptosis of dopaminergic cells in parkinson’s disease mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567705/ https://www.ncbi.nlm.nih.gov/pubmed/34736417 http://dx.doi.org/10.1186/s12868-021-00668-7 |
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