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Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effect...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567942/ https://www.ncbi.nlm.nih.gov/pubmed/34745433 http://dx.doi.org/10.1080/19768354.2021.1991472 |
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author | Kim, Dongwoo Koun, Soonil Kim, Seung Young Ha, Young Ran Choe, Jung Wan Jung, Sung Woo Hyun, Jong Jin Jung, Young Kul Koo, Ja Seol Yim, Hyung Joon Lee, Sang Woo |
author_facet | Kim, Dongwoo Koun, Soonil Kim, Seung Young Ha, Young Ran Choe, Jung Wan Jung, Sung Woo Hyun, Jong Jin Jung, Young Kul Koo, Ja Seol Yim, Hyung Joon Lee, Sang Woo |
author_sort | Kim, Dongwoo |
collection | PubMed |
description | Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effects. In this study, intestinal motility was monitored in zebrafish by tracking intestinal transit using fluorescence, after which an opioid-induced constipation model was established using loperamide. We then evaluated the prokinetic effect of diatrizoate meglumine (Gastrografin®), which has been empirically used to treat post-operative ileus or adhesive small bowel obstructions. Diatrizoate meglumine was effective in promoting bowel movements in an opioid-induced zebrafish constipation model and its prokinetic effect was associated with an increased expression of interstitial cells of Cajal (ICC) markers. Therefore, the loperamide-induced zebrafish constipation model developed herein is a promising tool to evaluate novel constipation therapies. |
format | Online Article Text |
id | pubmed-8567942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-85679422021-11-05 Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model Kim, Dongwoo Koun, Soonil Kim, Seung Young Ha, Young Ran Choe, Jung Wan Jung, Sung Woo Hyun, Jong Jin Jung, Young Kul Koo, Ja Seol Yim, Hyung Joon Lee, Sang Woo Anim Cells Syst (Seoul) Articles Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effects. In this study, intestinal motility was monitored in zebrafish by tracking intestinal transit using fluorescence, after which an opioid-induced constipation model was established using loperamide. We then evaluated the prokinetic effect of diatrizoate meglumine (Gastrografin®), which has been empirically used to treat post-operative ileus or adhesive small bowel obstructions. Diatrizoate meglumine was effective in promoting bowel movements in an opioid-induced zebrafish constipation model and its prokinetic effect was associated with an increased expression of interstitial cells of Cajal (ICC) markers. Therefore, the loperamide-induced zebrafish constipation model developed herein is a promising tool to evaluate novel constipation therapies. Taylor & Francis 2021-10-25 /pmc/articles/PMC8567942/ /pubmed/34745433 http://dx.doi.org/10.1080/19768354.2021.1991472 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Kim, Dongwoo Koun, Soonil Kim, Seung Young Ha, Young Ran Choe, Jung Wan Jung, Sung Woo Hyun, Jong Jin Jung, Young Kul Koo, Ja Seol Yim, Hyung Joon Lee, Sang Woo Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title | Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title_full | Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title_fullStr | Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title_full_unstemmed | Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title_short | Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model |
title_sort | prokinetic effects of diatrizoate meglumine (gastrografin®) in a zebrafish for opioid-induced constipation model |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567942/ https://www.ncbi.nlm.nih.gov/pubmed/34745433 http://dx.doi.org/10.1080/19768354.2021.1991472 |
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