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The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up

The first case of COVID-19 in Nigeria was recorded on February 27, 2020, being an imported case by an Italian expatriate, to the country. Since then, there has been steady increase in the number of cases. However, the number of cases in Nigeria is low in comparison to cases reported by other countri...

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Autores principales: Amoo, Olufemi Samuel, Adewara, Funmi, Tijani, Bosun, Onuigbo, Tochukwu Ifeanyi, Ikemefuna, Amaka Stephanie, Oraegbu, Joy Isioma, Rizvi, Tazeen, Okwuraiwe, Azuka, Onwuamah, Chika, Shaibu, Joseph, James, Ayorinde, Ohihoin, Greg, Ige, Fehintola, Kareithi, Dorcas, David, Agatha, Karera, Steven, Agboola, Hammed, Adeniyi, Anthony, Obi, Josephine, Achanya, Dominic, Odewale, Ebenezer, Oforomeh, Osaga, Liboro, Gideon, Nwogbe, Olayemi, Ezechi, Oliver, Adegbola, Richard, Audu, Rosemary, Salako, Babatunde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568151/
https://www.ncbi.nlm.nih.gov/pubmed/34735503
http://dx.doi.org/10.1371/journal.pone.0259398
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author Amoo, Olufemi Samuel
Adewara, Funmi
Tijani, Bosun
Onuigbo, Tochukwu Ifeanyi
Ikemefuna, Amaka Stephanie
Oraegbu, Joy Isioma
Rizvi, Tazeen
Okwuraiwe, Azuka
Onwuamah, Chika
Shaibu, Joseph
James, Ayorinde
Ohihoin, Greg
Ige, Fehintola
Kareithi, Dorcas
David, Agatha
Karera, Steven
Agboola, Hammed
Adeniyi, Anthony
Obi, Josephine
Achanya, Dominic
Odewale, Ebenezer
Oforomeh, Osaga
Liboro, Gideon
Nwogbe, Olayemi
Ezechi, Oliver
Adegbola, Richard
Audu, Rosemary
Salako, Babatunde
author_facet Amoo, Olufemi Samuel
Adewara, Funmi
Tijani, Bosun
Onuigbo, Tochukwu Ifeanyi
Ikemefuna, Amaka Stephanie
Oraegbu, Joy Isioma
Rizvi, Tazeen
Okwuraiwe, Azuka
Onwuamah, Chika
Shaibu, Joseph
James, Ayorinde
Ohihoin, Greg
Ige, Fehintola
Kareithi, Dorcas
David, Agatha
Karera, Steven
Agboola, Hammed
Adeniyi, Anthony
Obi, Josephine
Achanya, Dominic
Odewale, Ebenezer
Oforomeh, Osaga
Liboro, Gideon
Nwogbe, Olayemi
Ezechi, Oliver
Adegbola, Richard
Audu, Rosemary
Salako, Babatunde
author_sort Amoo, Olufemi Samuel
collection PubMed
description The first case of COVID-19 in Nigeria was recorded on February 27, 2020, being an imported case by an Italian expatriate, to the country. Since then, there has been steady increase in the number of cases. However, the number of cases in Nigeria is low in comparison to cases reported by other countries with similar large populations, despite the poor health system prevailing in the country. This has been mainly attributed to the low testing capacity in Nigeria among other factors. Therefore, there is a need for innovative ways to increase the number of persons testing for COVID-19. The aim of the study was to pilot a nasopharyngeal swab self-sample collection model that would help increase COVID-19 testing while ensuring minimal person-to-person contact being experienced at the testing center. 216 participants took part in this study which was carried out at the Nigerian Institute of Medical Research between June and July 2020. Amongst the 216 participants, 174 tested negatives for both self-collected samples and samples collected by Professionals, 30 tested positive for both arms, with discrepancies occurring in 6 samples where the self-collected samples were positive while the ones collected by the professionals were negative. The same occurred in another set of 6 samples with the self-collected samples being negative and the professional—collected sample coming out positive, with a sensitivity of 83.3% and a specificity of 96.7%. The results of the interrater analysis are Kappa = 0.800 (95% CI, 0.690 to 0.910) which implies an outstanding agreement between the two COVID-19 sampling methods. Furthermore, since p< 0.001 Kappa (k) coefficient is statistically different from zero, our findings have shown that self-collected samples can be reliable in the diagnosis of COVID-19.
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spelling pubmed-85681512021-11-05 The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up Amoo, Olufemi Samuel Adewara, Funmi Tijani, Bosun Onuigbo, Tochukwu Ifeanyi Ikemefuna, Amaka Stephanie Oraegbu, Joy Isioma Rizvi, Tazeen Okwuraiwe, Azuka Onwuamah, Chika Shaibu, Joseph James, Ayorinde Ohihoin, Greg Ige, Fehintola Kareithi, Dorcas David, Agatha Karera, Steven Agboola, Hammed Adeniyi, Anthony Obi, Josephine Achanya, Dominic Odewale, Ebenezer Oforomeh, Osaga Liboro, Gideon Nwogbe, Olayemi Ezechi, Oliver Adegbola, Richard Audu, Rosemary Salako, Babatunde PLoS One Research Article The first case of COVID-19 in Nigeria was recorded on February 27, 2020, being an imported case by an Italian expatriate, to the country. Since then, there has been steady increase in the number of cases. However, the number of cases in Nigeria is low in comparison to cases reported by other countries with similar large populations, despite the poor health system prevailing in the country. This has been mainly attributed to the low testing capacity in Nigeria among other factors. Therefore, there is a need for innovative ways to increase the number of persons testing for COVID-19. The aim of the study was to pilot a nasopharyngeal swab self-sample collection model that would help increase COVID-19 testing while ensuring minimal person-to-person contact being experienced at the testing center. 216 participants took part in this study which was carried out at the Nigerian Institute of Medical Research between June and July 2020. Amongst the 216 participants, 174 tested negatives for both self-collected samples and samples collected by Professionals, 30 tested positive for both arms, with discrepancies occurring in 6 samples where the self-collected samples were positive while the ones collected by the professionals were negative. The same occurred in another set of 6 samples with the self-collected samples being negative and the professional—collected sample coming out positive, with a sensitivity of 83.3% and a specificity of 96.7%. The results of the interrater analysis are Kappa = 0.800 (95% CI, 0.690 to 0.910) which implies an outstanding agreement between the two COVID-19 sampling methods. Furthermore, since p< 0.001 Kappa (k) coefficient is statistically different from zero, our findings have shown that self-collected samples can be reliable in the diagnosis of COVID-19. Public Library of Science 2021-11-04 /pmc/articles/PMC8568151/ /pubmed/34735503 http://dx.doi.org/10.1371/journal.pone.0259398 Text en © 2021 Amoo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Amoo, Olufemi Samuel
Adewara, Funmi
Tijani, Bosun
Onuigbo, Tochukwu Ifeanyi
Ikemefuna, Amaka Stephanie
Oraegbu, Joy Isioma
Rizvi, Tazeen
Okwuraiwe, Azuka
Onwuamah, Chika
Shaibu, Joseph
James, Ayorinde
Ohihoin, Greg
Ige, Fehintola
Kareithi, Dorcas
David, Agatha
Karera, Steven
Agboola, Hammed
Adeniyi, Anthony
Obi, Josephine
Achanya, Dominic
Odewale, Ebenezer
Oforomeh, Osaga
Liboro, Gideon
Nwogbe, Olayemi
Ezechi, Oliver
Adegbola, Richard
Audu, Rosemary
Salako, Babatunde
The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title_full The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title_fullStr The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title_full_unstemmed The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title_short The use of DIY (Do it yourself) sampling and telemonitoring model for COVID-19 qPCR testing scale up
title_sort use of diy (do it yourself) sampling and telemonitoring model for covid-19 qpcr testing scale up
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568151/
https://www.ncbi.nlm.nih.gov/pubmed/34735503
http://dx.doi.org/10.1371/journal.pone.0259398
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