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Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population

BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS...

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Autores principales: Othman, Galawezh Obaid, Mohammad, Nawsherwan Sadiq, Saeed, Chiman Hameed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568245/
https://www.ncbi.nlm.nih.gov/pubmed/34795724
http://dx.doi.org/10.4314/ahs.v21i2.26
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author Othman, Galawezh Obaid
Mohammad, Nawsherwan Sadiq
Saeed, Chiman Hameed
author_facet Othman, Galawezh Obaid
Mohammad, Nawsherwan Sadiq
Saeed, Chiman Hameed
author_sort Othman, Galawezh Obaid
collection PubMed
description BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS &METHODS: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520). RESULTS: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele. CONCLUSION: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimization of patient care.
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spelling pubmed-85682452021-11-17 Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population Othman, Galawezh Obaid Mohammad, Nawsherwan Sadiq Saeed, Chiman Hameed Afr Health Sci Articles BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS &METHODS: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520). RESULTS: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele. CONCLUSION: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimization of patient care. Makerere Medical School 2021-06 /pmc/articles/PMC8568245/ /pubmed/34795724 http://dx.doi.org/10.4314/ahs.v21i2.26 Text en © 2021 Othman GO et al. https://creativecommons.org/licenses/by/4.0/Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Othman, Galawezh Obaid
Mohammad, Nawsherwan Sadiq
Saeed, Chiman Hameed
Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title_full Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title_fullStr Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title_full_unstemmed Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title_short Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
title_sort molecular study of nucleophosmin 1(npm1) gene in acute myeloid leukemia in kurdish population
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568245/
https://www.ncbi.nlm.nih.gov/pubmed/34795724
http://dx.doi.org/10.4314/ahs.v21i2.26
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