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Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Makerere Medical School
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568245/ https://www.ncbi.nlm.nih.gov/pubmed/34795724 http://dx.doi.org/10.4314/ahs.v21i2.26 |
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author | Othman, Galawezh Obaid Mohammad, Nawsherwan Sadiq Saeed, Chiman Hameed |
author_facet | Othman, Galawezh Obaid Mohammad, Nawsherwan Sadiq Saeed, Chiman Hameed |
author_sort | Othman, Galawezh Obaid |
collection | PubMed |
description | BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS &METHODS: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520). RESULTS: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele. CONCLUSION: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimization of patient care. |
format | Online Article Text |
id | pubmed-8568245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Makerere Medical School |
record_format | MEDLINE/PubMed |
spelling | pubmed-85682452021-11-17 Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population Othman, Galawezh Obaid Mohammad, Nawsherwan Sadiq Saeed, Chiman Hameed Afr Health Sci Articles BACKGROUND: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population. PATIENTS &METHODS: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520). RESULTS: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele. CONCLUSION: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimization of patient care. Makerere Medical School 2021-06 /pmc/articles/PMC8568245/ /pubmed/34795724 http://dx.doi.org/10.4314/ahs.v21i2.26 Text en © 2021 Othman GO et al. https://creativecommons.org/licenses/by/4.0/Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Othman, Galawezh Obaid Mohammad, Nawsherwan Sadiq Saeed, Chiman Hameed Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title | Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title_full | Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title_fullStr | Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title_full_unstemmed | Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title_short | Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population |
title_sort | molecular study of nucleophosmin 1(npm1) gene in acute myeloid leukemia in kurdish population |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568245/ https://www.ncbi.nlm.nih.gov/pubmed/34795724 http://dx.doi.org/10.4314/ahs.v21i2.26 |
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