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Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements
Nucleic acid analysis is used in many areas of life sciences such as medicine, food safety, and environmental monitoring. Accurate, reliable measurements of nucleic acids are crucial for maximum impact, yet users are often unaware of the global metrological infrastructure that exists to support thes...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568362/ https://www.ncbi.nlm.nih.gov/pubmed/34738220 http://dx.doi.org/10.1007/s00216-021-03712-x |
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author | Milavec, Mojca Cleveland, Megan H. Bae, Young-Kyung Wielgosz, Robert I. Vonsky, Maxim Huggett, Jim F. |
author_facet | Milavec, Mojca Cleveland, Megan H. Bae, Young-Kyung Wielgosz, Robert I. Vonsky, Maxim Huggett, Jim F. |
author_sort | Milavec, Mojca |
collection | PubMed |
description | Nucleic acid analysis is used in many areas of life sciences such as medicine, food safety, and environmental monitoring. Accurate, reliable measurements of nucleic acids are crucial for maximum impact, yet users are often unaware of the global metrological infrastructure that exists to support these measurements. In this work, we describe international efforts to improve nucleic acid analysis, with a focus on the Nucleic Acid Analysis Working Group (NAWG) of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM). The NAWG is an international group dedicated to improving the global comparability of nucleic acid measurements; its primary focus is to support the development and maintenance of measurement capabilities and the dissemination of measurement services from its members: the National Metrology Institutes (NMIs) and Designated Institutes (DIs). These NMIs and DIs provide DNA and RNA measurement services developed in response to the needs of their stakeholders. The NAWG members have conducted cutting edge work over the last 20 years, demonstrating the ability to support the reliability, comparability, and traceability of nucleic acid measurement results in a variety of sectors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03712-x. |
format | Online Article Text |
id | pubmed-8568362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-85683622021-11-05 Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements Milavec, Mojca Cleveland, Megan H. Bae, Young-Kyung Wielgosz, Robert I. Vonsky, Maxim Huggett, Jim F. Anal Bioanal Chem Review Nucleic acid analysis is used in many areas of life sciences such as medicine, food safety, and environmental monitoring. Accurate, reliable measurements of nucleic acids are crucial for maximum impact, yet users are often unaware of the global metrological infrastructure that exists to support these measurements. In this work, we describe international efforts to improve nucleic acid analysis, with a focus on the Nucleic Acid Analysis Working Group (NAWG) of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM). The NAWG is an international group dedicated to improving the global comparability of nucleic acid measurements; its primary focus is to support the development and maintenance of measurement capabilities and the dissemination of measurement services from its members: the National Metrology Institutes (NMIs) and Designated Institutes (DIs). These NMIs and DIs provide DNA and RNA measurement services developed in response to the needs of their stakeholders. The NAWG members have conducted cutting edge work over the last 20 years, demonstrating the ability to support the reliability, comparability, and traceability of nucleic acid measurement results in a variety of sectors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03712-x. Springer Berlin Heidelberg 2021-11-04 2022 /pmc/articles/PMC8568362/ /pubmed/34738220 http://dx.doi.org/10.1007/s00216-021-03712-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Milavec, Mojca Cleveland, Megan H. Bae, Young-Kyung Wielgosz, Robert I. Vonsky, Maxim Huggett, Jim F. Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title | Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title_full | Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title_fullStr | Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title_full_unstemmed | Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title_short | Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
title_sort | metrological framework to support accurate, reliable, and reproducible nucleic acid measurements |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568362/ https://www.ncbi.nlm.nih.gov/pubmed/34738220 http://dx.doi.org/10.1007/s00216-021-03712-x |
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