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Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria
Aphanamixis polystachya may be a natural, renewable resource against antibiotic-resistant bacterial infections. The antibacterial activity of A. polystachya leaf and bark extracts was investigated against three antibiotic-resistant bacterial species and one fungus. Methanolic leaf extract showed onl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568816/ https://www.ncbi.nlm.nih.gov/pubmed/34764775 http://dx.doi.org/10.1016/j.sjbs.2021.07.032 |
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author | Paul, Gobindo Kumar Mahmud, Shafi Hasan, Md. Mehedi Zaman, Shahriar Uddin, Md. Salah Saleh, Md. Abu |
author_facet | Paul, Gobindo Kumar Mahmud, Shafi Hasan, Md. Mehedi Zaman, Shahriar Uddin, Md. Salah Saleh, Md. Abu |
author_sort | Paul, Gobindo Kumar |
collection | PubMed |
description | Aphanamixis polystachya may be a natural, renewable resource against antibiotic-resistant bacterial infections. The antibacterial activity of A. polystachya leaf and bark extracts was investigated against three antibiotic-resistant bacterial species and one fungus. Methanolic leaf extract showed only limited antibacterial activity but both methanolic and aqueous bark extract showed high antimicrobial activity. In an antioxidant activity test, leaf and bark extracts exhibited 50% free radical scavenging at a concentration of 107.14 ± 3.14 μg/mL and 97.13 ± 3.05 μg/mL, respectively, indicating that bark extracts offer more antioxidative activity than leaf extracts. Bark extracts also showed lower toxicity than leaf extracts. This suggests that bark extracts may offer greater development potential than leaf extracts. The molecular dynamics were also investigated through the simulated exploration of multiple potential interactions to understand the interaction dynamics (root-mean-square deviation, solvent-accessible surface area, radius of gyration, and the hydrogen bonding of chosen compounds to protein targets) and possible mechanisms of inhibition. This molecular modeling of compounds derived from A. polystachya revealed that inhibition may occur by binding to the active sites of the target proteins of the tested bacterial strains. A. polystachya bark extract may be used as a natural source of drugs to control antibiotic-resistant bacteria. |
format | Online Article Text |
id | pubmed-8568816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85688162021-11-10 Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria Paul, Gobindo Kumar Mahmud, Shafi Hasan, Md. Mehedi Zaman, Shahriar Uddin, Md. Salah Saleh, Md. Abu Saudi J Biol Sci Original Article Aphanamixis polystachya may be a natural, renewable resource against antibiotic-resistant bacterial infections. The antibacterial activity of A. polystachya leaf and bark extracts was investigated against three antibiotic-resistant bacterial species and one fungus. Methanolic leaf extract showed only limited antibacterial activity but both methanolic and aqueous bark extract showed high antimicrobial activity. In an antioxidant activity test, leaf and bark extracts exhibited 50% free radical scavenging at a concentration of 107.14 ± 3.14 μg/mL and 97.13 ± 3.05 μg/mL, respectively, indicating that bark extracts offer more antioxidative activity than leaf extracts. Bark extracts also showed lower toxicity than leaf extracts. This suggests that bark extracts may offer greater development potential than leaf extracts. The molecular dynamics were also investigated through the simulated exploration of multiple potential interactions to understand the interaction dynamics (root-mean-square deviation, solvent-accessible surface area, radius of gyration, and the hydrogen bonding of chosen compounds to protein targets) and possible mechanisms of inhibition. This molecular modeling of compounds derived from A. polystachya revealed that inhibition may occur by binding to the active sites of the target proteins of the tested bacterial strains. A. polystachya bark extract may be used as a natural source of drugs to control antibiotic-resistant bacteria. Elsevier 2021-11 2021-07-16 /pmc/articles/PMC8568816/ /pubmed/34764775 http://dx.doi.org/10.1016/j.sjbs.2021.07.032 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Paul, Gobindo Kumar Mahmud, Shafi Hasan, Md. Mehedi Zaman, Shahriar Uddin, Md. Salah Saleh, Md. Abu Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title | Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title_full | Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title_fullStr | Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title_full_unstemmed | Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title_short | Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria |
title_sort | biochemical and in silico study of leaf and bark extracts from aphanamixis polystachya against common pathogenic bacteria |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568816/ https://www.ncbi.nlm.nih.gov/pubmed/34764775 http://dx.doi.org/10.1016/j.sjbs.2021.07.032 |
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