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Overexpression of CXCL8 gene in Saudi colon cancer patients

Colorectal cancer (CRC) is one of the leading causes of death in Saudi Arabia. CRC mostly affects older age groups, but now a days it also appears frequently at a young age. However, the complete genetic etiology of CRC remains unknown. To identify the genetic factors responsible for this cancer typ...

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Detalles Bibliográficos
Autores principales: Rasool, Mahmood, Natesan Pushparaj, Peter, Karim, Sajjad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568844/
https://www.ncbi.nlm.nih.gov/pubmed/34764737
http://dx.doi.org/10.1016/j.sjbs.2021.09.031
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author Rasool, Mahmood
Natesan Pushparaj, Peter
Karim, Sajjad
author_facet Rasool, Mahmood
Natesan Pushparaj, Peter
Karim, Sajjad
author_sort Rasool, Mahmood
collection PubMed
description Colorectal cancer (CRC) is one of the leading causes of death in Saudi Arabia. CRC mostly affects older age groups, but now a days it also appears frequently at a young age. However, the complete genetic etiology of CRC remains unknown. To identify the genetic factors responsible for this cancer type and to search for biomarkers for early diagnosis and prevention, we collected sixteen CRC tumor tissue samples and six normal colon tissues and extracted mRNA and synthesized cDNA. We then performed microarray transcriptomic profiling of Saudi patients with colon cancer. Gene expression was analyzed using Partek Genomics Suite, and principal component analysis (PCA) was performed to separate the different clusters of colon cancer and healthy tissues. Distinct differences in gene expression profiles were observed between colon cancer and normal tissue samples. Subsequently, we validated gene expression using real-time PCR. We found that the C-X-C motif chemokine ligand 8 (CXCL8) gene was expressed most in CRC samples. CXCL8 expressed 25.6 folds more in CRC tissues than in healthy tissues. In conclusion, we found that CXCL8 is the chief biomarker gene that is expressed most in CRC and plays an important role in tumor progression and metastasis.
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spelling pubmed-85688442021-11-10 Overexpression of CXCL8 gene in Saudi colon cancer patients Rasool, Mahmood Natesan Pushparaj, Peter Karim, Sajjad Saudi J Biol Sci Original Article Colorectal cancer (CRC) is one of the leading causes of death in Saudi Arabia. CRC mostly affects older age groups, but now a days it also appears frequently at a young age. However, the complete genetic etiology of CRC remains unknown. To identify the genetic factors responsible for this cancer type and to search for biomarkers for early diagnosis and prevention, we collected sixteen CRC tumor tissue samples and six normal colon tissues and extracted mRNA and synthesized cDNA. We then performed microarray transcriptomic profiling of Saudi patients with colon cancer. Gene expression was analyzed using Partek Genomics Suite, and principal component analysis (PCA) was performed to separate the different clusters of colon cancer and healthy tissues. Distinct differences in gene expression profiles were observed between colon cancer and normal tissue samples. Subsequently, we validated gene expression using real-time PCR. We found that the C-X-C motif chemokine ligand 8 (CXCL8) gene was expressed most in CRC samples. CXCL8 expressed 25.6 folds more in CRC tissues than in healthy tissues. In conclusion, we found that CXCL8 is the chief biomarker gene that is expressed most in CRC and plays an important role in tumor progression and metastasis. Elsevier 2021-11 2021-09-17 /pmc/articles/PMC8568844/ /pubmed/34764737 http://dx.doi.org/10.1016/j.sjbs.2021.09.031 Text en © 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Rasool, Mahmood
Natesan Pushparaj, Peter
Karim, Sajjad
Overexpression of CXCL8 gene in Saudi colon cancer patients
title Overexpression of CXCL8 gene in Saudi colon cancer patients
title_full Overexpression of CXCL8 gene in Saudi colon cancer patients
title_fullStr Overexpression of CXCL8 gene in Saudi colon cancer patients
title_full_unstemmed Overexpression of CXCL8 gene in Saudi colon cancer patients
title_short Overexpression of CXCL8 gene in Saudi colon cancer patients
title_sort overexpression of cxcl8 gene in saudi colon cancer patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568844/
https://www.ncbi.nlm.nih.gov/pubmed/34764737
http://dx.doi.org/10.1016/j.sjbs.2021.09.031
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