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Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil

Impact of environmental perturbations i.e., nitrogen (N), phosphorus (P), potassium (K), and rice straw (Rs) on the dynamics of soil bacterial and archaeal communities are multifactor dependent and seeks a contemporary approach to study underlying mechanisms. The current study investigates the effec...

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Autores principales: Barq, Mohsin Gulzar, Mubashar Hassan, Muhammad, Yasmin, Humaira, shahzad, Asim, Malik, Noshaba Hassan, Lorenz, Nicola, Abdullah Alsahli, Abdulaziz, Dick, Richard P., Ali, Naeem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568845/
https://www.ncbi.nlm.nih.gov/pubmed/34764741
http://dx.doi.org/10.1016/j.sjbs.2021.08.091
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author Barq, Mohsin Gulzar
Mubashar Hassan, Muhammad
Yasmin, Humaira
shahzad, Asim
Malik, Noshaba Hassan
Lorenz, Nicola
Abdullah Alsahli, Abdulaziz
Dick, Richard P.
Ali, Naeem
author_facet Barq, Mohsin Gulzar
Mubashar Hassan, Muhammad
Yasmin, Humaira
shahzad, Asim
Malik, Noshaba Hassan
Lorenz, Nicola
Abdullah Alsahli, Abdulaziz
Dick, Richard P.
Ali, Naeem
author_sort Barq, Mohsin Gulzar
collection PubMed
description Impact of environmental perturbations i.e., nitrogen (N), phosphorus (P), potassium (K), and rice straw (Rs) on the dynamics of soil bacterial and archaeal communities are multifactor dependent and seeks a contemporary approach to study underlying mechanisms. The current study investigates the effect of pure and mixed fertilizers on soil physicochemical properties, the microbial community structure, and their functional metabolic predictions. It involved amendments with distinct combinations of N as C(H(2)N)(2)O, P and K as KH(2)PO(4), K as KCl, and Rs in paddy soil microcosms with concentrations common in rice fields agriculture. Soil pH, electrical conductivity (EC), total carbon (TC), total nitrogen (TN), organic matter (OM), available K (AK), and total extractable P (TEP) were evaluated. To comprehend community variation and functional predictions, 16S rRNA-based high throughput sequencing (HTS) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were employed, respectively. Our findings showed enhanced community richness and diversity in all amendments compared to control. Proteobacteria, Actinobacteria, and Firmicutes were dominant bacterial phyla. Regarding relative abundance, Chloroflexi, Bacteroidetes, and Verrucomicrobia showed positive while Actinobacteria, Acidobacteria, and Gemmatimonadetes showed negative trends compared to controls. Thaumarchaeota and Euryarchaeota were dominant archaeal phyla and exhibited increasing and decreasing trends, respectively. The PICRUSt analysis indicated functional prediction more towards amino acid, carbohydrate, energy, and lipid metabolism while less towards others. Concerning energy metabolism, most and least responsive treatments were KP and controls, respectively. These outcomes enhanced our understanding regarding soil quality, fertilizer composition and application, and functional metabolomics of archaea and bacteria.
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spelling pubmed-85688452021-11-10 Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil Barq, Mohsin Gulzar Mubashar Hassan, Muhammad Yasmin, Humaira shahzad, Asim Malik, Noshaba Hassan Lorenz, Nicola Abdullah Alsahli, Abdulaziz Dick, Richard P. Ali, Naeem Saudi J Biol Sci Original Article Impact of environmental perturbations i.e., nitrogen (N), phosphorus (P), potassium (K), and rice straw (Rs) on the dynamics of soil bacterial and archaeal communities are multifactor dependent and seeks a contemporary approach to study underlying mechanisms. The current study investigates the effect of pure and mixed fertilizers on soil physicochemical properties, the microbial community structure, and their functional metabolic predictions. It involved amendments with distinct combinations of N as C(H(2)N)(2)O, P and K as KH(2)PO(4), K as KCl, and Rs in paddy soil microcosms with concentrations common in rice fields agriculture. Soil pH, electrical conductivity (EC), total carbon (TC), total nitrogen (TN), organic matter (OM), available K (AK), and total extractable P (TEP) were evaluated. To comprehend community variation and functional predictions, 16S rRNA-based high throughput sequencing (HTS) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were employed, respectively. Our findings showed enhanced community richness and diversity in all amendments compared to control. Proteobacteria, Actinobacteria, and Firmicutes were dominant bacterial phyla. Regarding relative abundance, Chloroflexi, Bacteroidetes, and Verrucomicrobia showed positive while Actinobacteria, Acidobacteria, and Gemmatimonadetes showed negative trends compared to controls. Thaumarchaeota and Euryarchaeota were dominant archaeal phyla and exhibited increasing and decreasing trends, respectively. The PICRUSt analysis indicated functional prediction more towards amino acid, carbohydrate, energy, and lipid metabolism while less towards others. Concerning energy metabolism, most and least responsive treatments were KP and controls, respectively. These outcomes enhanced our understanding regarding soil quality, fertilizer composition and application, and functional metabolomics of archaea and bacteria. Elsevier 2021-11 2021-09-06 /pmc/articles/PMC8568845/ /pubmed/34764741 http://dx.doi.org/10.1016/j.sjbs.2021.08.091 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Barq, Mohsin Gulzar
Mubashar Hassan, Muhammad
Yasmin, Humaira
shahzad, Asim
Malik, Noshaba Hassan
Lorenz, Nicola
Abdullah Alsahli, Abdulaziz
Dick, Richard P.
Ali, Naeem
Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title_full Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title_fullStr Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title_full_unstemmed Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title_short Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
title_sort variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568845/
https://www.ncbi.nlm.nih.gov/pubmed/34764741
http://dx.doi.org/10.1016/j.sjbs.2021.08.091
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