circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3

The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detect...

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Autores principales: Huang, Yizhen, Zhang, Zhenlei, Wang, Jianle, Shen, Shuying, Yao, Teng, Xu, Yining, Chen, Zizheng, Fang, Bin, Ma, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568895/
https://www.ncbi.nlm.nih.gov/pubmed/34611269
http://dx.doi.org/10.1038/s12276-021-00674-z
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author Huang, Yizhen
Zhang, Zhenlei
Wang, Jianle
Shen, Shuying
Yao, Teng
Xu, Yining
Chen, Zizheng
Fang, Bin
Ma, Jianjun
author_facet Huang, Yizhen
Zhang, Zhenlei
Wang, Jianle
Shen, Shuying
Yao, Teng
Xu, Yining
Chen, Zizheng
Fang, Bin
Ma, Jianjun
author_sort Huang, Yizhen
collection PubMed
description The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene (ATP1B3) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3. The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.
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spelling pubmed-85688952021-11-17 circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3 Huang, Yizhen Zhang, Zhenlei Wang, Jianle Shen, Shuying Yao, Teng Xu, Yining Chen, Zizheng Fang, Bin Ma, Jianjun Exp Mol Med Article The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene (ATP1B3) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3. The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD. Nature Publishing Group UK 2021-10-06 /pmc/articles/PMC8568895/ /pubmed/34611269 http://dx.doi.org/10.1038/s12276-021-00674-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Huang, Yizhen
Zhang, Zhenlei
Wang, Jianle
Shen, Shuying
Yao, Teng
Xu, Yining
Chen, Zizheng
Fang, Bin
Ma, Jianjun
circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title_full circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title_fullStr circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title_full_unstemmed circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title_short circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
title_sort circspg21 protects against intervertebral disc disease by targeting mir-1197/atp1b3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568895/
https://www.ncbi.nlm.nih.gov/pubmed/34611269
http://dx.doi.org/10.1038/s12276-021-00674-z
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