Cargando…

Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis

Objective: Coronary heart disease (CHD) is a complex disease caused by multifaceted interaction between genetic and environmental factors, which makes identification of the most likely disease candidate proteins and their associated risk markers a big challenge. Atherosclerosis is presented by a bro...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Heyu, Ruan, Jianjun, Chen, Yanqiu, Yan, Zhaohan, Shi, Kaiyao, Li, Xiangdong, Yang, Ping, Meng, Fanbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569131/
https://www.ncbi.nlm.nih.gov/pubmed/34746269
http://dx.doi.org/10.3389/fcvm.2021.758035
_version_ 1784594585509429248
author Meng, Heyu
Ruan, Jianjun
Chen, Yanqiu
Yan, Zhaohan
Shi, Kaiyao
Li, Xiangdong
Yang, Ping
Meng, Fanbo
author_facet Meng, Heyu
Ruan, Jianjun
Chen, Yanqiu
Yan, Zhaohan
Shi, Kaiyao
Li, Xiangdong
Yang, Ping
Meng, Fanbo
author_sort Meng, Heyu
collection PubMed
description Objective: Coronary heart disease (CHD) is a complex disease caused by multifaceted interaction between genetic and environmental factors, which makes identification of the most likely disease candidate proteins and their associated risk markers a big challenge. Atherosclerosis is presented by a broad spectrum of heart diseases, including stable coronary artery disease (SCAD) and acute myocardial infarction (AMI), which is the progressive stage of SCAD. As such, the correct and prompt diagnosis of atherosclerosis turns into imperative for precise and prompt disease diagnosis, treatment and prognosis. Methods: The current work aims to look for specific protein markers for differential diagnosis of coronary atherosclerosis. Thirty male patients between 45 and 55 years diagnosed with atherosclerosis were analyzed by tandem mass tag (TMT) mass spectrometry. The study excluded those who were additionally diagnosed with hypertension and type 1 and 2 diabetes. The Mufuzz analysis was applied to select target proteins for precise and prompt diagnosis of atherosclerosis, most of which were most related to high lipid metabolism. The parallel reaction monitoring (PRM) was used to verify the selected target proteins. Finally, The receiver operating characteristic curve (ROC) was calculated by a random forest experiment. Results: One thousand one hundred and forty seven proteins were identified in the TMT mass spectrometry, 907 of which were quantifiable. In the PRM study, six proteins related to lipid metabolism pathway were selected for verification and they were ALB, SHBG, APOC2, APOC3, APOC4, SAA4. Conclusion: Through the detected specific changes in these six proteins, our results provide accuracy in atherosclerosis patients' diagnosis, especially in cases with varying types of the disease.
format Online
Article
Text
id pubmed-8569131
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85691312021-11-06 Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis Meng, Heyu Ruan, Jianjun Chen, Yanqiu Yan, Zhaohan Shi, Kaiyao Li, Xiangdong Yang, Ping Meng, Fanbo Front Cardiovasc Med Cardiovascular Medicine Objective: Coronary heart disease (CHD) is a complex disease caused by multifaceted interaction between genetic and environmental factors, which makes identification of the most likely disease candidate proteins and their associated risk markers a big challenge. Atherosclerosis is presented by a broad spectrum of heart diseases, including stable coronary artery disease (SCAD) and acute myocardial infarction (AMI), which is the progressive stage of SCAD. As such, the correct and prompt diagnosis of atherosclerosis turns into imperative for precise and prompt disease diagnosis, treatment and prognosis. Methods: The current work aims to look for specific protein markers for differential diagnosis of coronary atherosclerosis. Thirty male patients between 45 and 55 years diagnosed with atherosclerosis were analyzed by tandem mass tag (TMT) mass spectrometry. The study excluded those who were additionally diagnosed with hypertension and type 1 and 2 diabetes. The Mufuzz analysis was applied to select target proteins for precise and prompt diagnosis of atherosclerosis, most of which were most related to high lipid metabolism. The parallel reaction monitoring (PRM) was used to verify the selected target proteins. Finally, The receiver operating characteristic curve (ROC) was calculated by a random forest experiment. Results: One thousand one hundred and forty seven proteins were identified in the TMT mass spectrometry, 907 of which were quantifiable. In the PRM study, six proteins related to lipid metabolism pathway were selected for verification and they were ALB, SHBG, APOC2, APOC3, APOC4, SAA4. Conclusion: Through the detected specific changes in these six proteins, our results provide accuracy in atherosclerosis patients' diagnosis, especially in cases with varying types of the disease. Frontiers Media S.A. 2021-10-22 /pmc/articles/PMC8569131/ /pubmed/34746269 http://dx.doi.org/10.3389/fcvm.2021.758035 Text en Copyright © 2021 Meng, Ruan, Chen, Yan, Shi, Li, Yang and Meng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Meng, Heyu
Ruan, Jianjun
Chen, Yanqiu
Yan, Zhaohan
Shi, Kaiyao
Li, Xiangdong
Yang, Ping
Meng, Fanbo
Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title_full Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title_fullStr Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title_full_unstemmed Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title_short Investigation of Specific Proteins Related to Different Types of Coronary Atherosclerosis
title_sort investigation of specific proteins related to different types of coronary atherosclerosis
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569131/
https://www.ncbi.nlm.nih.gov/pubmed/34746269
http://dx.doi.org/10.3389/fcvm.2021.758035
work_keys_str_mv AT mengheyu investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT ruanjianjun investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT chenyanqiu investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT yanzhaohan investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT shikaiyao investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT lixiangdong investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT yangping investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis
AT mengfanbo investigationofspecificproteinsrelatedtodifferenttypesofcoronaryatherosclerosis